1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Angiopoietins
  4. ANG-1
  5. ANGPT1/Angiopoietin-1, Rat (P. pastoris, His)

ANGPT1/Angiopoietin-1, Rat (P. pastoris, His)

目录号: HY-P700560
产品使用指南

在大鼠中,ANGPT1(血管生成素-1)激活 TIE2 受体,诱导酪氨酸磷酸化并影响与 VEGF 不同的内皮发育过程。ANGPT1 在介导内皮、基质和间质之间的相互作用中发挥着至关重要的作用。它参与血管成熟,强调其在血管发育中的重要性,在早期心脏发育中具有潜在作用。ANGPT1/Angiopoietin-1, Rat (P. pastoris, His) 是重组的 ANGPT1/Angiopoietin-1,由 P. pastoris 酵母表达,带有 N-6*His 标签。ANGPT1/Angiopoietin-1, Rat (P. pastoris, His) 全长 478 个氨基酸,分子量为 57.4 kDa。

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描述

In rats, ANGPT1 (Angiopoietin-1) activates the TIE2 receptor, inducing tyrosine phosphorylation and influencing endothelial developmental processes distinct from VEGF. ANGPT1 plays a crucial role in mediating reciprocal interactions between endothelium, matrix, and mesenchyme. It participates in blood vessel maturation, emphasizing its significance in vascular development, with a potential role in early heart development. ANGPT1/Angiopoietin-1, Rat (P. pastoris, His) is the recombinant rat-derived ANGPT1/Angiopoietin-1, Rat, expressed by P. pastoris, with N-6*His labeled tag. The total length of ANGPT1/Angiopoietin-1, Rat (P. pastoris, His) is 478 a.a., with molecular weight of 57.4 kDa.

研究背景

ANGPT1,在大鼠中也称为血管生成素-1,通过结合并激活 TIE2 受体,诱导其酪氨酸磷酸化来发挥作用。该蛋白参与内皮发育过程,与 VEGF 的作用不同,并且似乎在内皮与周围基质和间质之间的相互作用中发挥着至关重要的作用。此外,ANGPT1 参与介导血管成熟和稳定性,表明其在血管发育中的重要性。值得注意的是,它可能在心脏的早期发育中发挥重要作用。

种属

Rat

表达系统

P. pastoris

标签

N-6*His

蛋白编号

O35460 (S20-F497)

基因 ID
蛋白结构
N-term
6*His
ANGPT1 (S20-F497)
Accession # O35460
C-term
同用名
angiopoietin 1; angiopoietin-1; Ang1; KIAA0003; ANG-1; AGP1; AGPT; ANG1;
氨基酸序列

SNQRRSPENGGRRYNRIQHGQCAYTFILPEHDGNCRESATEQYNTNALQRDAPHVETDFSSQKLQHLEHVMENYTQWLQKLENYIVENMKSEMAQIQQNAVQNHTATMLEIGTSLLSQTAEQTRKLTDVETQVLNQTSRLEIQLLENSLSTYELEKQLLQQTNEILKIQEKNSLLEHKILEMEGKHKEELDTLKEEKENLQGLVTRQTFIIQELEKQLSRATSNNSVLQKQQLELMDTVHNLVSLCTKEVLLKGGKREEEKPFRDCADVYQAGFNKSGIYTIYFNNMPEPKKVFCNMDVNEGGWTVIQHREDGSLDFQRGWKEYKMGFGNPSGEYWLGNEFIFAITSQRQYMLRIELMDWEGNRAYSQYDRFHIGNQKQNYRLYLKGHTGTAGKQSSLILHGADFSTKDADNDNCMCKCALMLTGGWWFDACGPSNLNGMFYTAGQNHGKLNGIKWHYFKGPSYSLRSTTMMIRPLDF

分子量

57.4 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ANGPT1/Angiopoietin-1, Rat (P. pastoris, His) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ANGPT1/Angiopoietin-1, Rat (P. pastoris, His)
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HY-P700560
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