Animal-Free BMP-15 蛋白, Human (His)

Bone morphogenetic protein 15 (BMP-15; GDF9B), also known as growth differentiation factor 9B (GDF9B), is a polymorphic ligand protein of the TGFβ family and is only expressed in follicular cells. BMP15 is closely related to GDF9 and synergistically regulates the genetic development of follicles. BMP15 is involved in p38 MAPK and HIF-1α/SCF signaling pathway, respectively, and can up-regulate the expression of anti-Mullerian hormone (AMH) and polycystic ovarian syndrome (PCOS) related stem cell factor (SCF) in granulosa cells. The total length of human BMP-15 protein is 392 amino acids (M1-R392), with 4 glycosylation domains. Animal-Free BMP-15 Protein, Human (His) has a total length of 125 amino acids (Q268-R392), is expressed in E. coli cells with a C-terminal His-tag.

骨形态发生蛋白 15（BMP-15；GDF9B）也称为生长和分化因子 9B (GDF9B)，是一种属于 TGFβ 家族的多态性配体蛋白，仅在卵母细胞中表达^[1] .
BMP15 与 GDF9 密切相关，GDF9 对于早期卵巢卵泡发生至关重要^[1]。
BMP15 和 GDF9 参与卵泡发育的遗传控制。其主要功能包括调节细胞增殖/分化、卵泡存活/闭锁和卵母细胞成熟，以创造支持卵泡选择和生长的环境^[2]。
BMP15 参与 p38 MAPK 通路上调颗粒细胞中抗苗勒管激素 (AMH) 的表达，该激素由窦前和小窦卵泡的颗粒细胞 (GC) 产生，在调节原始卵泡的募集和 FSH 依赖性卵泡发育中发挥作用< super>[3]。
另外，BMP15 结合 HIF-1α/SCF 信号通路诱导多囊卵巢综合征 (PCOS) 相关卵泡的人 GC 中干细胞因子 (SCF) 表达^[4]。
BMP-15广泛存在于不同的动物中，而人类的序列与大鼠（63.66%）和小鼠（64.01）不同。

BMP-15 (huamn; 500 ng/mL; 48 h) induces FSHR expression in HGrC1 cells^[5].
BMP-15 (huamn; 500 ng/mL; 0-90 min) increases phosphorylation of Smad 1/5/8 in HGrC1 cells^[5].

Human

E. coli

C-His

O95972 (Q268-R392)

9210  [NCBI]

MAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR

Approximately 14.88 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<0.1 EU per 1 μg of the protein by the LAL method.

• [1]. Galloway SM, et al. Bmp15 mutations and ovarian function. Mol Cell Endocrinol. 2002 May 31;191(1):15-8.  [Content Brief]

  [2]. Liu MN, et al. The role of BMP15 and GDF9 in the pathogenesis of primary ovarian insufficiency. Hum Fertil (Camb). 2021 Dec;24(5):325-332.  [Content Brief]

  [3]. Zhao Z, et al. BMP15 regulates AMH expression via the p38 MAPK pathway in granulosa cells from goat. Theriogenology. 2018 Sep 15;118:72-79.  [Content Brief]

  [4]. Cao LY, et al. Aberrant BMP15/HIF-1α/SCF signaling pathway in human granulosa cells is involved in the PCOS related abnormal follicular development. Gynecol Endocrinol. 2022 Sep 23:1-7.  [Content Brief]

  [5]. Shimizu K, et al. Molecular mechanism of FSHR expression induced by BMP15 in human granulosa cells. J Assist Reprod Genet. 2019 Jun;36(6):1185-1194.  [Content Brief]