Animal-Free Noggin 蛋白, Human (His)

Noggin protein is an important BMP inhibitor that plays an indispensable role in the neural tube, somite growth, cartilage morphogenesis, and joint formation. Its homodimeric structure promotes significant interactions with GDF5 and possibly GDF6, inhibiting chondrocyte differentiation. Animal-Free Noggin Protein, Human (His) is the recombinant human-derived animal-FreeNoggin protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free Noggin Protein, Human (His) is 205 a.a., with molecular weight of ~24 kDa.

Noggin 蛋白是骨形态发生蛋白 (BMP) 信号传导复杂领域中的关键抑制剂，在神经管和体节生长中发挥着不可或缺的作用，并有助于软骨形态发生和关节形成的复杂过程。通过其同二聚体结构，Noggin 与 GDF5（可能还有 GDF6）建立了显着的相互作用，对软骨细胞分化发挥抑制作用。这种分子相互作用强调了 Noggin 在调节胚胎发育关键方面的关键地位，强调了它在塑造对正常生长和形态发生至关重要的复杂模式和结构方面的微妙参与。

Measure by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is <0.05 µg/mL in the presence of 50 ng/mL of recombinant human BMP-4.

Human

E. coli

C-His

Q13253 (Q28-C232)

9241  [NCBI]

MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGGQRCGWIPIQYPIISECKCSC

Approximately 24 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 0.1% sarkosyl in 1X PBS, pH 8.0, trehalose.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.