AQP4 蛋白, Mouse (Cell-Free, His)

The AQP4 protein forms water-specific channels that are critical for brain water homeostasis and lymphoid solute transport. It regulates water exchange at the blood-brain interface, promoting the influx of cerebrospinal fluid into the brain and the drainage of interstitial fluid. AQP4 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP4 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of AQP4 Protein, Mouse (Cell-Free, His) is 323 a.a., with molecular weight of 40.5 kDa.

AQP4 蛋白形成水特异性通道，对于脑水稳态和类淋巴溶质运输至关重要。它在血脑界面的正常水交换率中起着至关重要的作用，并且对于脑脊液流入大脑皮层和实质以及间质液的排出是必需的。AQP4 对于清除脑间质液中的溶质（包括可溶性 β-淀粉样肽）至关重要。此外，它在尿水稳态和浓缩能力方面发挥着重要作用。AQP4 四聚体以同源四聚体形式存在，可以在膜中形成寡聚阵列，不同组织之间的尺寸不同。相邻细胞中 AQP4 寡聚阵列之间的相互作用有助于细胞间粘附，它是包含 MLC1、TRPV4、HEPACAM 和 ATP1B1 的复合物的一部分。

Mouse

E. coli Cell-free

N-10*His

P55088 (M1-V323)

11829

MSDGAAARRWGKCGHSCSRESIMVAFKGVWTQAFWKAVSAEFLATLIFVLLGVGSTINWGGSENPLPVDMVLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYIIAQCLGAIIGAGILYLVTPPSVVGGLGVTTVHGNLTAGHGLLVELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGASMNPARSFGPAVIMGNWANHWIYWVGPIMGAVLAGALYEYVFCPDVELKRRLKEAFSKAAQQTKGSYMEVEDNRSQVETEDLILKPGVVHVIDIDRGEEKKGKDSSGEVLSSV

Observed band size: Monomer: 38 kDa Dimer: 76 kDa It is speculated that the protein forms a dimeric structure.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% Brij-78, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.