ATP6V1F 蛋白, Human

ATP6V1F is an important subunit of the vacuolar (H+)-ATPase (V-ATPase) V1 complex and is an important component of this enzyme's role in cellular pH regulation. It forms a catalytic AB heterodimer and peripheral stem that plays a role in ATP hydrolysis. ATP6V1F Protein, Human is the recombinant human-derived ATP6V1F protein, expressed by E. coli , with tag free. The total length of ATP6V1F Protein, Human is 119 a.a., with molecular weight of ~13 KDa.

ATP6V1F 是液泡 (H+)-ATP 酶 (V-ATP 酶) V1 复合物的重要亚基，构成多亚基酶的一部分，在细胞 pH 调节中发挥核心作用。该酶是一种异多聚体组装体，包含两个基本复合体：ATP 水解 V1 复合体和质子易位 V0 复合体。在 V1 复合体中，ATP6V1F 有助于形成催化 AB 异二聚体，构成异六聚体，外周茎由 EG 异二聚体组成。此外，它是中心转子的一个组成部分，与亚基 D 协同工作。V1 复合体负责 ATP 水解，而 V0 复合体（其中未直接提及但暗示了 ATP6V1F）对于质子易位至关重要。膜。这种质子运输涉及各种亚基，包括质子运输亚基a、蛋白脂质亚基环c9c''、旋转亚基d、亚基e和f以及附属亚基ATP6AP1/Ac45和ATP6AP2/PRR。这些亚基的协同作用强调了 ATP6V1F 在 V-ATP 酶复杂机制中的重要性，V-ATP 酶酸化并维持细胞区室中的 pH 值，在某些细胞类型中，酸化和维持质膜的 pH 值，从而影响各种生理过程。

Measured by its binding ability in a functional ELISA. When Recombinant Human ATP6V1F is present at 2 μg/mL, can bind ATP6V1F antibody. The ED₅₀ for this effect is 195 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Human ATP6V1F is present at 2 μg/mL, can bind ATP6V1F antibody.The ED₅₀ for this effect is 195 ng/mL.

Human

E. coli

Tag Free

Q16864 ( M1-R119 )

9296

MAGRGKLIAVIGDEDTVTGFLLGGIGELNKNRHPNFLVVEKDTTINEIEDTFRQFLNRDDIGIILINQYIAEMVRHALDAHQQSIPAVLEIPSKEHPYDAAKDSILRRARGMFTAEDLR

Approximately 13 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.