1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. B3GAT3 蛋白, Human (N-His)

B3GAT3 蛋白, Human (N-His)

目录号: HY-P700269
产品使用指南

B3GAT3 蛋白是葡萄糖醛酸转移酶基因家族的重要成员,催化糖胺聚糖-蛋白质连接形成的最后一步,这对于蛋白聚糖生物合成非常重要。该酶表现出严格的受体特异性,识别非还原末端糖和异头连接。它在大脑、淋巴结和其他 25 个组织中的广泛表达强调了它在不同生理环境中的重要性。B3GAT3 蛋白, Human (N-His) 是重组的 B3GAT3 蛋白,由 E. coli 表达,带有 N-His 标签。

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描述

B3GAT3 Proteins, a crucial member of the glucuronyltransferase gene family, catalyze the final step of glycosaminoglycan-protein linkage formation, important in proteoglycan biosynthesis.This enzyme exhibits strict acceptor specificity, recognizing nonreducing terminal sugars and anomeric linkages.Its widespread expression in tissues like the brain, lymph nodes, and 25 others emphasizes its significance in diverse physiological contexts.B3GAT3 Protein, Human (N-His) is the recombinant human-derived B3GAT3 protein, expressed by E.coli , with N-His labeled tag.

研究背景

B3GAT3 是葡萄糖醛酸转移酶基因家族的成员,通过催化糖胺聚糖-蛋白质连接形成的最后一步,在蛋白聚糖的生物合成中发挥着至关重要的作用。该酶表现出严格的受体特异性,识别非还原性末端糖及其异头连接。已在 3 号染色体上鉴定出与 B3GAT3 相关的假基因。B3GAT3 的表达广泛,在各种组织中普遍存在,包括大脑、淋巴结和 25 个其他组织,强调了其在不同生理环境中的重要性。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

NP_036332.2 (E72-V335)

基因 ID
蛋白结构
N-term
His
B3GAT3 (E72-V335)
Accession # NP_036332.2
C-term
同用名
rHuB3GAT3, His; B3GAT3; Beta-1,3-Glucuronyltransferase 3;
分子量

Approximately 33 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

B3GAT3 Protein, Human (N-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
B3GAT3 Protein, Human (N-His)
目录号:
HY-P700269
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