pro-Beta-NGF 蛋白, Human (223a.a)

Pro-Beta-NGF protein plays a crucial role in the development and maintenance of the nervous system by binding to NTRK1 and NGFR receptors. Immature NGF precursors act as ligands for the SORCS2-NGFR complex, triggering signaling pathways affecting RAC1/2, the actin cytoskeleton, and neuronal growth. pro-Beta-NGF Protein, Human (223a.a) is the recombinant human-derived pro-Beta-NGF protein, expressed by E. coli , with tag free. The total length of pro-Beta-NGF Protein, Human (223a.a) is 223 a.a., with molecular weight of ~30 kDa.

pro-Beta-NGF 蛋白在交感神经和感觉神经系统的发育和维持中起着至关重要的作用。作为细胞外配体，它与 NTRK1 和 NGFR 受体结合，启动调节神经元增殖、分化和存活的信号级联。值得注意的是，未成熟的 NGF 前体 (proNGF) 作为 SORCS2-NGFR 异二聚体受体复合物的配体，激活信号通路，导致 RAC1 和/或 RAC2 失活、肌动蛋白细胞骨架重组和神经元生长锥塌陷。与成熟的 NGF 相比，proNGF 在体外促进神经元凋亡。此外，pro-Beta-NGF 对血小板糖蛋白 VI 的金属蛋白酶依赖性蛋白水解具有抑制作用。该蛋白质与溶血磷脂酰肌醇和溶血磷脂酰丝氨酸（脂质结合的和无脂质的）相互作用，有助于肥大细胞释放组胺。pro-Beta-NGF 的同二聚体结构与 NTRK1、NGFR 和 SORCS2 相互作用，介导对细胞过程的各种下游影响。此外，pro-Beta-NGF 与 SORT1 和 NGFR 形成的受体复合物结合，导致 NGF 内吞作用。这些复杂的相互作用凸显了 pro-Beta-NGF 在协调神经元功能和细胞反应中的多方面作用。

Human

E. coli

Tag Free

P01138 (E19-A241)

4803  [NCBI]

EPHSESNVPAGHTIPQAHWTKLQHSLDTALRRARSAPAAAIAARVAGQTRNITVDPRLFKKRRLRSPRVLFSTQPPREAADTQDLDFEVGGAAPFNRTHRSKRSSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAVRRA

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.