BPGM 蛋白, Human (His)

BPGM Protein, Human (His) expresses in E. coliwith a His tag. Bisphosphoglycerate mutase is an erythrocyte-specific enzyme catalyzing a series of intermolecular phosphoryl group transfer reactions. Its main function is to synthesize 2,3-bisphosphoglycerate, the allosteric effector of hemoglobin^[1].

双磷酸甘油酸变位酶（BPGM）是一种红细胞特异性三功能酶，催化一系列分子间磷酰基转移反应。BPGM 的主要活性是合酶，将 1,3-二磷酸甘油酸 (1,3-BPG) 转化为 2,3-二磷酸甘油酸 (2,3-BPG)。第二个活性是变位酶，催化 2-磷酸甘油酸 (2-PGA) 和 3-磷酸甘油酸 (3-PGA) 之间的相互转化。第三个活性是磷酸酶，将 2,3-BPG 水解为 3-PGA 或 2-PGA 和磷酸盐。BPGM 存在于红细胞和胎盘细胞中，它调节 2,3-BPG^[2] 的水平。

Human

E. coli

C-6*His

P07738 (S2-K259)

669  [NCBI]

SKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGMEEARNCGKQLKALNFEFDLVFTSVLNRSIHTAWLILEELGQEWVPVESSWRLNERHYGALIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDVLERLLPYWNERIAPEVLRGKTILISAHGNSSRALLKHLEGISDEDIINITLPTGVPILLELDENLRAVGPHQFLGDQEAIQAAIKKVEDQGKVKQAKKHHHHHH

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. Wang Y, et al. Seeing the process of histidine phosphorylation in human bisphosphoglycerate mutase. J Biol Chem. 2006;281(51):39642-39648.  [Content Brief]

  [2]. Chu WT, et al. Insights into the phosphatase and the synthase activities of human bisphosphoglycerate mutase: a quantum mechanics/molecular mechanics simulation. Phys Chem Chem Phys. 2014;16(9):3946-3954.  [Content Brief]