CD9 蛋白, Human (HEK293, His)

CD9 protein is an integral membrane protein that plays a critical regulatory role in processes such as sperm-egg fusion, platelet activation, and cell adhesion. CD9 is critical for sperm-egg fusion, coordinating multi-protein complexes on the oocyte surface. CD9 Protein, Human (HEK293, His) is the recombinant human-derived CD9 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CD9 Protein, Human (HEK293, His) is 84 a.a., with molecular weight of ~10 kDa.

CD9 是一种完整的膜蛋白，在调节各种生物过程中发挥着关键作用，包括精卵融合、血小板活化和聚集以及细胞粘附。CD9 位于卵母细胞的细胞表面，对于精卵融合至关重要，可能通过协调融合过程所需的多蛋白复合物和膜形态来实现。在成肌细胞中，CD9 与 CD81 和 PTGFRN 结合，抑制肌肉再生过程中的肌管融合。在巨噬细胞内，CD9 与 CD81 和 beta-1/beta-2 整合素结合，防止巨噬细胞融合成专门摄取补体调理大颗粒的多核巨细胞。CD9 还阻碍单核细胞祖细胞融合成负责骨吸收的破骨细胞。除了在融合事件中的作用外，CD9 还充当 PSG17 的受体，并与血小板活化、聚集、节旁连接形成、细胞粘附、细胞运动和肿瘤转移有关。CD9 形成二硫键连接的同源二聚体和高级同源寡聚体，以及与其他四跨膜蛋白家族成员的异源寡聚体。它与整合素 ITGAV:ITGB3 和 ITGA6:ITGB1 相互作用，并且是单核细胞/巨噬细胞膜中整合素-四跨膜蛋白复合物的一部分。CD9 与 CD63、PTGFRN、IGSF8、CR2/CD21 和 PDPN 形成复合物，在各种细胞功能和相互作用中发挥着至关重要的作用。

Human

HEK293

C-6*His

P21926 (S112-I195)

928  [NCBI]

SHKDEVIKEVQEFYKDTYNKLKTKDEPQRETLKAIHYALNCCGLAGGVEQFISDICPKKDVLETFTVKSCPDAIKEVFDNKFHI

Approximately 10 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.