Coagulation Factor X/F10 蛋白, Mouse (HEK293, His)

Coagulation Factor X/F10 Protein, a crucial vitamin K-dependent glycoprotein, is central to blood clotting. It produces Factor Xa, essential for converting prothrombin to thrombin, a process facilitated by factor Va, calcium, and phospholipid in the intricate cascade of blood coagulation. Coagulation Factor X/F10 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Coagulation Factor X/F10 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Coagulation Factor X/F10 Protein, Mouse (HEK293, His) is 461 a.a., with molecular weight of ~55.0 kDa.

凝血因子 X/F10 蛋白是一种维生素 K 依赖性糖蛋白，在血液凝固中发挥着关键作用。这种蛋白质产生的 Xa 因子有助于将凝血酶原转化为凝血酶。这种转化发生在因子 Va、钙和磷脂存在的情况下，从而促进了复杂的凝血过程。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

C-6*His

O88947 (G21-N481)

14058  [NCBI]

GKGVFINRERANNVLARTRRANSFFEEFKKGNLERECMEEICSYEEVREIFEDDEKTKEYWTKYKDGDQCESSPCQNQGACRDGIGGYTCTCSEGFEGKNCELFVRKLCRLDNGDCDQFCREEQNSVVCSCASGYFLGNDGKSCISTAPFPCGKITTGRRKRSVALNTSDSELDLEDALLDEDFLSPTENPIELLNLNETQPERSSDDLVRIVGGRECKDGECPWQALLINEDNEGFCGGTILNEFYILTAAHCLHQARRFKVRVGDRNTEKEEGNEMVHEVDVVIKHNKFQRDTYDYDIAVLRLKTPITFRMNVAPACLPQKDWAESTLMTQKTGIVSGFGRTHEKGRQSNILKMLEVPYVDRNTCKLSTSFSITQNMFCAGYEAKLEDACQGDSGGPHVTRFKNTYYVTGIVSWGEGCARKGKYGIYTKVTTFLKWIDRSMKARVGPTAETPRTAGPPN

50-60 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM MES, 150 mM NaCl, 1 mM CaCl₂, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.