CTRB1 蛋白, Human (HEK293, His)

Chymotrypsinogen B (CTRB1) is the precursor of the pancreatic proteolytic enzymes. CTRB1 is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate the functional enzyme. CTRB1 enables serine-type endopeptidase activity, being involved in response to apoptotic signal, nutrient, cytokine and peptide hormone. CTRB1 is located in lysosome and is a biomarker of pancreatitis. CTRB1 Protein, Human (HEK293, His) is the recombinant human-derived CTRB1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CTRB1 Protein, Human (HEK293, His) is 245 a.a., with molecular weight of 28-30 kDa.

胰凝乳蛋白酶原 B (CTRB1) 是丝氨酸蛋白酶家族的一员，是胰腺蛋白水解酶的主要前体。CTRB1 在胰腺的腺泡细胞中合成，并分泌到小肠中，在小肠中进行蛋白水解活化，产生功能性酶。CTRB1 激活丝氨酸型内肽酶活性，参与对凋亡信号、营养物质、细胞因子和肽类激素的反应。CTRB1 通过 Bid 切割活性诱导细胞凋亡，因为截断的 Bid 可导致线粒体快速释放细胞色素 C。CTRB1 位于溶酶体中，是胰腺炎的一个生物标志物。CTRB1 基因编码不同的异构体，这些异构体可能经历类似的加工以产生成熟的蛋白质^{[1][2][3][4][5]}。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P17538 (C19-N263)

1504  [NCBI]

CGVPAIHPVLSGLSRIVNGEDAVPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHCGVRTSDVVVAGEFDQGSDEENIQVLKIAKVFKNPKFSILTVNNDITLLKLATPARFSQTVSAVCLPSADDDFPAGTLCATTGWGKTKYNANKTPDKLQQAALPLLSNAECKKSWGRRITDVMICAGASGVSSCMGDSGGPLVCQKDGAWTLVGIVSWGSDTCSTSSPGVYARVTKLIPWVQKILAAN

28-30 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.