DHH 蛋白, Human

The DHH protein is cleaved into N- and C-product fragments through autoproteolysis and cholesterol transferase activity, where the N-product acquires the cholesterol moiety. In the endoplasmic reticulum, DHH is critical for juxtocrine signaling, endothelial integrity, and Schwann cell-mediated nerve sheath development. DHH Protein, Human (Tag Free) is the recombinant human-derived DHH protein, expressed by E. coli , with tag free. The total length of DHH Protein, Human (Tag Free) is 175 a.a., with molecular weight of ~20 kDa.

DHH 蛋白前体在其 C 末端区域表现出自蛋白水解和胆固醇转移酶活性，导致全长蛋白裂解为 N 产物和 C 产物片段，并在新生成的 N 产物中添加胆固醇部分。产品。这些过程发生在内质网中。DHH 在细胞间介导的邻分泌信号传导中发挥着至关重要的作用，并促进内皮完整性。它与 PTCH1 受体结合，与 SMO 结合，激活内皮细胞中靶基因的转录。在雪旺细胞中，DHH 控制周围神经鞘的发育以及间充质细胞向神经周管上皮样结构的转变。脂化 DHH N-产物对于各种发育模式事件、与 PTCH1 结合并激活靶基因转录至关重要。DHH 对于正常睾丸发育、精子发生、成年型间质细胞的形成以及管周细胞和生精小管的发育至关重要。此外，DHH 通过旁分泌机制激活邻近瓣膜间质细胞中的初级纤毛信号传导，并可能诱导外侧神经管中的运动神经元，同时防止 DHH 蛋白前体与 PTCH1 结合。

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.729 µg/mL, corresponding to a specific activity is 5.817×10² U/mg.

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  Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.729 µg/mL, corresponding to a specific activity is 5.817×10² U/mg.

Human

E. coli

Tag Free

O43323 (G24-G198)

50846

GPGRGPVGRRRYARKQLVPLLYKQFVPGVPERTLGASGPAEGRVARGSERFRDLVPNYNPDIIFKDEENSGADRLMTERCKERVNALAIAVMNMWPGVRLRVTEGWDEDGHHAQDSLHYEGRALDITTSDRDRNKYGLLARLAVEAGFDWVYYESRNHVHVSVKADNSLAVRAGG

Approximately 20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.