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  4. DLK-1 蛋白, Cynomolgus (HEK293, His)

DLK-1 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P700706
产品使用指南

DLK-1 也称为前脂肪细胞因子，是一种具有表皮生长因子 (EGF) 样重复序列的跨膜分泌蛋白。DLK-1通过控制 Wnt 信号传导调节先锋轴突的生长。DLK-1 是在包括肝细胞癌在内的许多癌症中表达的细胞表面蛋白，可能是单克隆抗体治疗癌症的潜在靶点。DLK-1 蛋白, Cynomolgus (HEK293, His) 是重组的 DLK-1 蛋白，由 HEK293 表达，带有 C-His 标签。DLK-1 蛋白, Cynomolgus (HEK293, His) 全长 283 个氨基酸，分子量为 42-52 kDa。

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描述

DLK-1, also known as pre adipokine, is a transmembrane-secreted protein with an epidermal growth factor (EGF) -like repeat sequence. DLK-1 regulates the growth of pioneer axons by controlling Wnt signaling. DLK-1 is a cell surface protein expressed in many cancers, including hepatocellular carcinoma, and maybe a potential target for monoclonal antibodies to treat cancer. DLK-1 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived DLK-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of DLK-1 Protein, Cynomolgus (HEK293, His) is 283 a.a., with molecular weight of 42-52 kDa.
研究背景

DLK-1 也称为前脂肪细胞因子，是一种具有表皮生长因子 (EGF) 样重复序列的跨膜分泌蛋白。DLK-1 是保守的 MAPKKK 双亮氨酸拉链激酶 (DLKs) 的成员，在突触形成、轴突再生和退化中起作用。DLK-1通过控制 Wnt 信号传导调节先锋轴突的生长。DLK-1 是在包括肝细胞癌在内的许多癌症中表达的细胞表面蛋白，可能是单克隆抗体治疗癌症的潜在靶点。
种属

Cynomolgus
表达系统

HEK293
标签

C-His
蛋白编号

XP_005562275.1 (A24-C306)
基因 ID
蛋白结构
N-term
DLK-1 (A24-C306)
Accession # XP_005562275.1
His
C-term
同用名
pG2; FA1; DLK; DLK1; DLK-1; Pref1; secredeltin; ZOG
分子量

42-52 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DLK-1 Protein, Cynomolgus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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