ELANE 蛋白, Human (GST)

ELANE is a serine protease that critically regulates natural killer cells, monocytes, and granulocytes by inhibiting C5a-dependent neutrophil enzyme release and chemotaxis, thereby shaping immune responses. It also inhibits pyroptosis by cleaving GSDMB, affecting the regulation of programmed cell death. ELANE Protein, Human (GST) is the recombinant human-derived ELANE protein, expressed by E. coli , with N-GST labeled tag. The total length of ELANE Protein, Human (GST) is 238 a.a., with molecular weight of ~52.6 kDa.

ELANE 是一种丝氨酸蛋白酶，在调节自然杀伤细胞、单核细胞和粒细胞的功能中发挥着至关重要的作用。它通过抑制 C5a 依赖性中性粒细胞酶释放和趋化性发挥作用，突出其在免疫反应中的调节作用。此外，ELANE 通过促进 GSDMB 的裂解参与抑制焦亡，表明其参与程序性细胞死亡的调节。值得注意的是，ELANE 通过在体外有效杀死大肠杆菌而表现出杀菌活性，同时不伤害金黄色葡萄球菌。这种抗菌作用归因于其消化大肠杆菌和肺炎克雷伯菌中的外膜蛋白 A (ompA) 的能力，强调了其在细菌防御机制中的作用。

Measured by its ability to cleave the fluorogenic peptide substrate, MeOSuc-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin (MeOSuc-AAPV-AMC). The specific activity is 1776.266 pmol/min/µg, as measured under the described conditions.

Human

E. coli

N-GST

P08246 (I30-H267)

1991  [NCBI]

IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNLSRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGVQCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCNGLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQRSEDNPCPHPRDPDPASRTH

Approximately 52.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.