ENTE 蛋白, S. aureus (N-His)

ENTE Proteinas are staphylococcal enterotoxins that activate the host immune system by binding to major histocompatibility complex class II and T-cell receptor (TCR) molecules. This interaction forms a ternary complex that activates T lymphocytes and induces the systemic release of proinflammatory cytokines. ENTE Protein, S. aureus (N-His) is the recombinant Staphylococcus aureus-derived ENTE protein, expressed by E. coli , with N-6*His labeled tag. The total length of ENTE Protein, S. aureus (N-His) is 230 a.a., with molecular weight of ~27 kDa.

ENTE 是一种葡萄球菌肠毒素，通过作为未加工的分子与主要组织相容性复合物 II 类和 T 细胞受体 (TCR) 分子结合，在激活宿主免疫系统中发挥关键作用。这种相互作用导致三元复合物的形成，进而激活大量 T 淋巴细胞，引发促炎细胞因子的全身释放。除了其免疫调节功能外，ENTE 还与葡萄球菌食物中毒综合征的发生有关，这种疾病的特征是中毒、发烧和胃肠道不适等症状。这些活动强调了 ENTE 对免疫反应途径和病理结果的影响。

Staphylococcus aureus

E. coli

N-6*His

P12993 (S28-T257)

/

SEEINEKDLRKKSELQRNALSNLRQIYYYNEKAITENKESDDQFLENTLLFKGFFTGHPWYNDLLVDLGSKDATNKYKGKKVDLYGAYYGYQCAGGTPNKTACMYGGVTLHDNNRLTEEKKVPINLWIDGKQTTVPIDKVKTSKKEVTVQELDLQARHYLHGKFGLYNSDSFGGKVQRGLIVFHSSEGSTVSYDLFDAQGQYPDTLLRIYRDNKTINSENLHIDLYLYTT

Approximately 27 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration, please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.