1. 重组蛋白
  2. CD Antigens
  3. Stem Cell CD Proteins Erythrocyte CD Proteins
  4. Glycophorin-A/CD235a
  5. GYPA/CD235a 蛋白, Mouse (HEK293, His)

GYPA/CD235a 蛋白, Mouse (HEK293, His)

目录号: HY-P77954
产品使用指南

GYPA/CD235a蛋白是锚蛋白1复合物的重要组成部分,它维持红细胞膜的稳定性和形状。作为主要的内在膜蛋白,GYPA 确保这些血细胞的结构完整性和功能。GYPA/CD235a 蛋白, Mouse (HEK293, His) 是重组的 GYPA/CD235a 蛋白,由 HEK293 表达,带有 C-His 标签。GYPA/CD235a 蛋白, Mouse (HEK293, His) 全长 108 个氨基酸,分子量为 47-55 kDa。

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描述

The GYPA/CD235a protein is an important component of the ankyrin 1 complex, which maintains the stability and shape of the red blood cell membrane. As a major intrinsic membrane protein, GYPA ensures the structural integrity and function of these blood cells. GYPA/CD235a Protein, Mouse (HEK293, His) is the recombinant mouse-derived GYPA/CD235a protein, expressed by HEK293 , with C-His labeled tag. The total length of GYPA/CD235a Protein, Mouse (HEK293, His) is 108 a.a., with molecular weight of 47-55 kDa.

研究背景

GYPA 是锚蛋白 1 复合物的重要组成部分,复杂地参与维持红细胞膜的稳定性和形状。GYPA 作为红细胞的主要内在膜蛋白,在这些血细胞的结构完整性和功能中发挥着至关重要的作用。N 末端糖基化片段突出于红细胞膜外,含有 MN 血型受体,强调了其参与血型决定因素。此外,GYPA 对于 SLC4A1 的正常功能至关重要,它的存在对于 SLC4A1 的高活性至关重要。此外,GYPA 还可作为流感病毒、恶性疟原虫红细胞结合抗原 175 (EBA-175) 和甲型肝炎病毒 (HAV) 的受体。值得注意的是,它形成同二聚体,是锚蛋白 1 复合物的关键成分,与其他蛋白质(如 ANK1、RHCE、RHAG、SLC4A1、EPB42、GYPB 和 AQP1)协同作用,维持红细胞膜结构。与 SLC4A1 的相互作用导致异四聚体的形成,突出了 GYPA 在红细胞结构分子组织中的协同作用。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P14220 (M1-V108)

基因 ID
蛋白结构
N-term
GYPA (M1-V108)
Accession # P14220
His
C-term
同用名
Glycophorin-A; PAS-2; CD235a; glycophorin Erik; glycophorin MiI; glycophorin MiIII; glycophorin MiV; glycophorin MiX; glycophorin SAT; GPA; GPErik; GpMiIII; GPSAT; GYPA; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); MNS; CD235a; MN
分子量

47-55 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GYPA/CD235a Protein, Mouse (HEK293, His)
目录号:
HY-P77954
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