IFI30 蛋白, Human (HEK293, His)

IFI30 is a lysosomal thiol reductase that plays a critical role in reducing protein disulfide bonds in the lysosomal environment. This enzymatic activity is critical for complete unfolding of proteins for lysosomal degradation. IFI30 Protein, Human (HEK293, His) is the recombinant human-derived IFI30 protein, expressed by HEK293 , with C-His labeled tag. The total length of IFI30 Protein, Human (HEK293, His) is 217 a.a., with molecular weight of 31-34 kDa.

IFI30 是一种溶酶体硫醇还原酶，在溶酶体环境中蛋白质二硫键还原中发挥着关键作用。这种酶活性对于溶酶体降解蛋白质的完全展开至关重要。值得注意的是，IFI30 在抗原加工中发挥着关键作用，特别有助于通过内吞还原从含有二硫键的抗原生成 MHC II 类限制性表位。此外，IFI30 还参与促进 CD8+ T 细胞对具有二硫键的外源抗原的 MHC I 类限制性识别，从而有助于交叉呈递过程。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-His

P13284 (S27-K243)

10437  [NCBI]

SPLQALDFFGNGPPVNYKTGNLYLRGPLKKSNAPLVNVTLYYEALCGGCRAFLIRELFPTWLLVMEILNVTLVPYGNAQEQNVSGRWEFKCQHGEEECKFNKVEACVLDELDMELAFLTIVCMEEFEDMERSLPLCLQLYAPGLSPDTIMECAMGDRGMQLMHANAQRTDALQPPHEYVPWVTVNGKPLEDQTQLLTLVCQLYQGKKPDVCPSSTSS

31-34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.