IL-12 蛋白, Cynomolgus (HEK293, His)

IL-12 Protein, forming a heterodimer with IL23A, generates the cytokine IL-23, pivotal in innate and adaptive immunity. Collaborating with IL-17, IL-23 prompts an acute response to infection in peripheral tissues. Binding to the receptor complex IL12RB1 and IL23R, IL-23 activates Jak-Stat signaling, preferentially stimulates memory T-cells, and induces pro-inflammatory cytokine production. Implicated in autoimmune inflammation and tumorigenesis, IL-23 acts as a growth factor for activated T and NK cells, enhances NK cell lytic activity, and stimulates IFN-gamma production by resting PBMC. IL-12 Protein, Cynomolgus (HEK293, His) is a recombinant protein dimer complex containing cynomolgus-derived IL-12 protein, expressed by HEK293, with C-His labeled tag. IL-12 Protein, Cynomolgus (HEK293, His), has molecular weight of 45-48 kDa (IL-12B) & 38-44 kDa (IL-12A), respectively.

IL-12 蛋白与 IL23A 形成异二聚体，从而产生 IL-23 白细胞介素，这是一种在先天免疫和适应性免疫中具有多种功能的细胞因子。IL-23 与 IL-17 合作，可以协调外周组织对感染的急性反应。IL-23 与由 IL12RB1 和 IL23R 组成的异二聚体受体复合物结合，激活 Jak-Stat 信号级联，优先刺激记忆 T 细胞而不是幼稚 T 细胞，并促进促炎细胞因子的产生。此外，IL-23 与诱导自身免疫性炎症有关，可能在自身免疫性炎症疾病和肿瘤发生中发挥作用。该细胞因子充当激活的 T 和 NK 细胞的生长因子，增强 NK/淋巴因子激活的杀伤细胞的裂解活性，并刺激静息 PBMC 产生 IFN-γ。

1.Immobilized Cynomolgus IL-12, His Tag at 0.5 μg/mL(100 μl/well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC₅₀ of <8.5 ng/mL determined by ELISA.
2.Measured by its ability to enhance IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 0.7733 ng/mL, corresponding to a specific activity is 1.293×10⁶ U/mg.

Cynomolgus

HEK293

C-His

G7P6S2 (I23-S328)&XP_005546300.2 (R57-S253)

/&102115316

A1:IWELKKDVYV
VELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSGEVLGSGKTLTIQVKEFGDAGQYTCHKGGEALSHSLLLLHKKEDGIWSTDVLKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSNPQGVTCGAVTLSAERVRGDNKEYEYSVECQEDSACPAAEERLPIEVMVDAIHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCIQVQGKSKREKKDRIFTDKTSATVICRKNASFSVQAQDRYYSSSWSEWASVPCS
A2:RNLSVATPGP
EMFPCLHHSQNLLKAASNTLQKARQILEFYPCTSEEIDHEDITKDKTSTVEACLPLELIKNESCLNSRETSFITNGSCLASRKTSFMMALCLRSIYEDLKMYQVEFKTMNAKLLRDPKRQIFLDQNILGVIDELMQALNFNSETVPQKSSLEEPDFYKTKIKLCILLHAFRIRAVTIDRVMSYLNAS

45-48 kDa (IL-12B) & 38-44 kDa (IL-12A)

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.