IMPA3 蛋白, Mouse (HEK293, His)

IMPA3 protein has 3'-nucleotidase activity and can hydrolyze PAP into AMP and phosphate. This function suggests a role in the formation of bone components during endochondral ossification by scavenging PAP produced by Golgi sulfotransferases. IMPA3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IMPA3 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of IMPA3 Protein, Mouse (HEK293, His) is 306 a.a., with molecular weight of ~38.0 kDa.

IMPA3 蛋白具有 3'-核苷酸酶活性，可特异性地将腺苷 3',5'-二磷酸 (PAP) 水解为腺苷 5'-单磷酸 (AMP) 和磷酸盐。这种酶功能表明在软骨内骨化衍生的骨骼成分形成中具有潜在作用，可能是通过清除糖胺聚糖硫酸化过程中高尔基磺基转移酶产生的腺苷3',5'-二磷酸。值得注意的是，IMPA3 对 3'-磷酸腺苷 5'-磷酸硫酸盐 (PAPS) 或磷酸肌醇 (IP) 底物（包括 I(1)P、I(1,4)P2、I(1,3,4)P3）没有活性、I(1,4,5)P3 和 I(1,3,4,5)P4。IMPA3 的选择性酶谱强调了它在核苷酸代谢中的特定参与及其在骨骼发育过程中的潜在调节作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

N-6*His

Q80V26 (E51-H356)

242291  [NCBI]

EVASDGGTVDLREMLAVAVLAAERGGDEVRRVRESNVLHEKSKGKTREGADDKMTSGDVLSNRKMFYLLKTAFPNVQINTEEHVDASDKEVIVWNRKIPEDILKEIAAPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPVLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPKIIVSRSHAGMVKQVALQTFGNQTSIIPAGGAGYKVLALLDVPDMTQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLNGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKSGH

Approximately 38.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.