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  3. Lactoferrin/LTF 蛋白, Mouse (HEK293, His)

Lactoferrin/LTF 蛋白, Mouse (HEK293, His)

目录号: HY-P74789
COA 产品使用指南

乳铁蛋白 (LTF) 是外分泌液中的主要铁结合蛋白,通过螯合铁和破坏细菌膜而表现出多种抗菌作用。它可以防止生物膜形成,影响骨代谢,并影响腺病毒和乳头瘤病毒感染。Lactoferrin/LTF 蛋白, Mouse (HEK293, His) 是重组的 乳铁蛋白/LTF 蛋白,由 HEK293 表达,带有 C-His 标签。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Lactoferrin (LTF) is a major iron-binding protein in exocrine fluid and exhibits multiple antibacterial effects by chelating iron and disrupting bacterial membranes.It prevents biofilm formation, affects bone metabolism, and affects adenovirus and papillomavirus infections.Lactoferrin/LTF Protein, Mouse (HEK293, His) is the recombinant mouse-derived Lactoferrin/LTF protein, expressed by HEK293 , with C-His labeled tag.

研究背景

乳铁蛋白 (LTF) 是一种主要的铁结合多功能蛋白,存在于母乳和粘膜分泌物等外分泌液中,具有多种生物活性。LTF 以其抗菌特性而闻名,它通过隔离游离铁并从细菌外膜释放脂多糖来阻碍微生物生长,从而发挥抑菌和杀菌作用。此外,它在防止铜绿假单胞菌感染中细菌生物膜的形成方面发挥作用,并对白色念珠菌表现出较弱的抗真菌活性。除了抗菌功能外,LTF还通过对成骨细胞发挥合成代谢、分化和抗凋亡作用来影响骨代谢,同时还抑制破骨细胞生成。它通过促进 C 种腺病毒与上皮细胞的结合来促进腺病毒感染,并抑制乳头瘤病毒感染。LTF 刺激 TLR4 信号传导通路,导致 NF-κ-B 激活和促炎细胞因子产生,同时干扰脂多糖刺激的 TLR4 信号传导。此外,它对中性粒细胞迁移具有抑制作用,并促进 VEGFA 介导的内皮细胞迁移和增殖。LTF 的多功能性延伸至其结合能力,包括与肝素、硫酸软骨素、肺炎球菌表面蛋白 A、溶菌酶和 DNA 的相互作用。

生物活性

Measured by its ability to increase the expression of TNF-α in THP-1 cells. The ED50 for this effect is 37.15 ng/mL, corresponding to a specific activity is 2.69×104 U/mg.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

AAH06904 (T22-Q707)

基因 ID
蛋白结构
N-term
LTF (T22-Q707)
Accession # AAH06904
His
C-term
同用名
Lactotransferrin; Lactoferrin; Kaliocin-1; Lactoferroxin-A; LTF
氨基酸序列

TTVRWCAVSNSEEEKCLRWQNEMRKVGGPPLSCVKKSSTRQCIQAIVTNRADAMTLDGGTMFDAGKPPYKLRPVAAEVYGTKEQPRTHYYAVAVVKNSSNFHLNQLQGLRSCHTGIGRSAGWKIPIGTLRPYLNWNGPPASLEEAVSKFFSKSCVPGAQKDRFPNLCSSCAGTGANKCASSPEEPYSGYAGALRCLRDNAGDVAFTRGSTVFEELPNKAERDQYKLLCPDNTWKPVTEYKECHLAQVPSHAVVSRSTNDKEEAIWELLRQSQEKFGKKQASGFQLFASPSGQKDLLFKESAIGFVRVPQKVDVGLYLTFSYTTSIQNLNKKQQDVIASKARVTWCAVGSEEKRKCDQWNRASRGRVTCISFPTTEDCIVAIMKGDADAMSLDGGYIYTAGKCGLVPVLAENQKSSKSNGLDCVNRPVEGYLAVAAVRREDAGFTWSSLRGKKSCHTAVDRTAGWNIPMGLLANQTRSCKFNEFFSQSCAPGADPKSNLCALCIGDEKGENKCAPNSKERYQGYTGALRCLAEKAGNVAFLKDSTVLQNTDGKNTEEWARNLKLKDFELLCLDDTRKPVTEAKNCHLAIAPNHAVVSRTDKVEVLQQVLLDQQVQFGRNGQRCPGEFCLFQSKTKNLLFNDNTECLAKIPGKTTSEKYLGKEYVIATERLKQCSSSPLLEACAFLTQ

分子量

Approximately 70 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization) or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Lactoferrin/LTF Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Lactoferrin/LTF Protein, Mouse (HEK293, His)
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