1. 重组蛋白
  2. Complement System
  3. MASP-2
  4. MASP2 蛋白, Mouse (His)

MASP2 蛋白, Mouse (His)

目录号: HY-P77800
产品使用指南

MASP2 蛋白是一种血清蛋白酶,通过自动催化裂解来裂解 C2 和 C4,在激活补体系统中发挥关键作用。这种激活导致 C3 转化酶的形成,这是通过甘露糖结合凝集素启动补体级联的关键步骤。MASP2 蛋白, Mouse (His) 是重组的 MASP2 蛋白,由 E. coli 表达,带有 C-His 标签。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

MASP2 Protein, a serum protease, plays a pivotal role in activating the complement system by cleaving C2 and C4 upon auto-catalytic cleavage.This activation leads to the formation of C3 convertase, a crucial step in initiating the complement cascade through mannose-binding lectin.MASP2 Protein, Mouse (His) is the recombinant mouse-derived MASP2 protein, expressed by E.coli , with C-His labeled tag.

研究背景

MASP2 是一种血清蛋白酶,对于通过甘露糖结合凝集素激活补体系统至关重要。在自动催化裂解后,MASP2 有效裂解 C2 和 C4,导致它们激活并随后形成 C3 转化酶,这对于补体级联至关重要。

种属

Mouse

表达系统

E. coli

标签

C-His

蛋白编号

Q91WP0 (T287-F685)

基因 ID
蛋白结构
N-term
MASP2 (T287-F685)
Accession # Q91WP0
His
C-term
同用名
MAP19; MASP-2; MASP1P1; sMap
分子量

18-20 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

MASP2 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MASP2 Protein, Mouse (His)
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