Matriptase/ST14 Catalytic Domain 蛋白, Human (His)

The Matriptase/ST14 catalytic domain protein originates from epithelial cells, forms a complex with HAI-1, and is activated by sphingosine-1-phosphate. It cleaves and activates hepatocyte growth factor/scatter factor and urokinase plasminogen activator, suggesting its role as an activator of other proteases and potential growth factors. Matriptase/ST14 Catalytic Domain Protein, Human (His) is the recombinant human-derived Matriptase/ST14 Catalytic Domain protein, expressed by E. coli , with N-6*His labeled tag.

Matriptase/ST14 催化域蛋白是一种源自上皮细胞的整合膜丝氨酸蛋白酶。它与 Kunitz 型丝氨酸蛋白酶抑制剂 HAI-1 形成复合物，并被 1-磷酸鞘氨醇激活。该蛋白酶已被证明能够裂解并激活肝细胞生长因子/散射因子以及尿激酶纤溶酶原激活剂，表明其作为上皮膜上其他蛋白酶和潜在生长因子的激活剂的作用。这种蛋白酶的表达与乳腺癌、结肠癌、前列腺癌和卵巢肿瘤有关，表明它参与癌症的侵袭和转移。它在结肠 (RPKM 103.0)、小肠 (RPKM 72.7) 和 15 个其他组织中广泛表达。

Measured by its ability to cleave the fluorogenic peptide substrate BOC-Gln-Ala-Arg-AMC (HY-134432A) that incubate at room temperature for 5 min. The specific activity is 43374.15 pmol/min/µg.

Human

E. coli

N-6*His

NP_068813 (G596-V855)

6768

GSDEKDCDCGLRSFTRQARVVGGTDADEGEWPWQVSLHALGQGHICGASLISPNWLVSAAHCYIDDRGFRYSDPTQWTAFLGLHDQSQRSAPGVQERRLKRIISHPFFNDFTFDYDIALLELEKPAEYSSMVRPICLPDASHVFPAGKAIWVTGWGHTQYGGTGALILQKGEIRVINQTTCENLLPQQITPRMMCVGFLSGGVDSCQGDSGGPLSSVEADGRIFQAGVVSWGDGCAQRNKPGVYTRLPLFRDWIKENTGV

Approximately 28 kDa (major) and minor auto-activation fragments

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCL, 150 mM NaCl, pH 7.5, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.