1. 重组蛋白
  2. Viral Proteins
  3. Influenza Viruses Proteins
  4. Influenza Virus Neuraminidase
  5. NA/Neuraminidase 蛋白, H4N6 (ABI47998, sf9, His)

NA/Neuraminidase 蛋白, H4N6 (ABI47998, sf9, His)

目录号: HY-P74722
产品使用指南

NA/Neuraminidase 蛋白是一种受体破坏酶,可从细胞受体上裂解末端唾液酸,通过靶向气道上皮细胞粘蛋白上的唾液酸部分,可能促进病毒入侵上呼吸道。NA 通过催化从病毒和细胞糖复合物中去除末端唾液酸残基,在病毒传播中发挥关键作用。此外,晚期内体/溶酶体运输中的唾液酸酶活性似乎增强了病毒复制。NA/Neuraminidase 蛋白, H4N6 (ABI47998, sf9, His) 是重组的 NA/神经氨酸酶蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。

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描述

NA/Neuraminidase Protein is a receptor-destroying enzyme that cleaves terminal sialic acids from cellular receptors, potentially facilitating viral invasion of the upper airways by targeting sialic acid moieties on airway epithelial cell mucin.NA plays a pivotal role in viral propagation by catalyzing the removal of terminal sialic acid residues from both viral and cellular glycoconjugates.Moreover, the sialidase activity in late endosome/lysosome traffic appears to enhance virus replication.NA/Neuraminidase Protein, H4N6 (ABI47998, sf9, His) is the recombinant Virus-derived NA/Neuraminidase protein, expressed by Sf9 insect cells , with N-His labeled tag.

研究背景

神经氨酸酶 (NA) 通过催化从病毒和细胞糖复合物中去除末端唾液酸残基,在病毒传播中发挥关键作用。具体来说,在病毒出芽过程中,NA 从糖基化血凝素 (HA) 上切下末端唾液酸,促进病毒颗粒的释放,并使病毒能够在循环中有效传播。通过防止自我聚集并确保从细胞表面去除唾液酸,NA 使子代病毒能够在细胞之间有效传播,从而避免单轮复制的限制。NA 被描述为一种受体破坏酶,可从细胞受体上裂解末端唾液酸,通过靶向气道上皮细胞粘蛋白上的唾液酸部分,可能促进病毒入侵上呼吸道。它在细胞内运输过程中与脂筏的关联,以及它对出芽的潜在的脂筏独立效应,凸显了 NA 在决定宿主范围限制、复制和毒力方面的多方面作用。此外,晚期内体/溶酶体运输中的唾液酸酶活性似乎增强了病毒复制[1][2][3]

种属

Virus

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

ABI47998 (H36-K470)

基因 ID

/

蛋白结构
N-term
His
H4N6 NA (H36-K470)
Accession # ABI47998
C-term
同用名
Influenza A H4N6 (A/mallard/Ohio/657/2002) Neuraminidase / NA Protein (His)
分子量

Approximately 50.2 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NA/Neuraminidase Protein, H4N6 (ABI47998, sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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NA/Neuraminidase Protein, H4N6 (ABI47998, sf9, His)
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HY-P74722
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