Neuropilin-1 蛋白, Rat (HEK293, His)

Neuropilin-1 protein is a cell surface receptor that plays multiple roles in cardiovascular development, angiogenesis, and neuronal circuit formation. It mediates chemical repulsion by recognizing ligands with CendR motifs, leading to internalization and vascular leakage. Neuropilin-1 Protein, Rat (HEK293, His) is the recombinant rat-derived Neuropilin-1 protein, expressed by HEK293 , with C-6*His labeled tag and K811R, P812-G828 delet.

Neuropilin-1 蛋白是一种细胞表面受体，在心血管系统发育、血管生成、神经元回路形成和器官发生中发挥多种作用。它充当信号蛋白化学脉冲活性的介体，并识别其配体上的 C 端规则 (CendR) 基序 R/KXXR/K，导致细胞内化和血管渗漏。它与多种配体结合，包括信号蛋白 3A、PGF 的 PLGF-2 同种型、VEGFA 的 VEGF165 同种型和 VEGFB。当与 KDR 共表达时，它增强 VEGF165 与 KDR 的结合并促进趋化性。Neuropilin-1 调节 VEGF 诱导的血管生成，并在运动神经元轴突引导和细胞体迁移中发挥至关重要的作用，特别是在胚胎发育中面部运动神经元的尾部迁移过程中。它还通过与 ABCB8/MITOSUR 相互作用参与线粒体铁转运的调节。Neuropilin-1 与 NRP2 形成同二聚体和异二聚体，与线粒体中的 PLXNB1、FER、VEGFA 和 ABCB8/MITOSUR 相互作用。

Measured by its binding ability in a functional ELISA. Immobilized Rat Neuropilin-1 at 2 µg/mL can bind Human VEGF165. The ED₅₀ for this effect is 0.8699 nM.

Rat

HEK293

C-6*His

Q9QWJ9 (F22-D854, K811R, P812-G828 del)

246331

FRSDKCGGTIKIENPGYLTSPGYPHSYHPSEKCEWLIQAPEPYQRIMINFNPHFDLEDRDCKYDYVEVIDGENEGGRLWGKFCGKIAPSPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTAPTGVIKSPGFPEKYPNSLECTYIIFAPKMSEIILEFESFDLEQDSNPPGGVFCRYDRLEIWDGFPEVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSISEDFKCMEALGMESGEIHSDQITASSQYGTNWSVERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYRVDISSNGEDWITLKEGNKAIIFQGNTNPTDVVFGVFPKPLITRFVRIKPASWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITASNQGDRNWMPENIRLVTSRTGWALPPSPHPYINEWLQVDLGDEKIVRGVIIQGGKHRENKVFMRKFKIAYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRAFTPLSTRFIRIYPERATHSGLGLRMELLGCEVEVPTAGPTTPNGNPVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTIIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDSHAQLRWRVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQSSAHCMTFWYHMSGSHVGTLRVKLHYQKPEEYDQLVWMVVGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHIPQEDCARSTPGYEEGKGDKNISRKPGNVLKTLD

Approximately 98-130 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.