NKp30/NCR3 蛋白, Cynomolgus/Rhesus Macaque (HEK293, His)

NKp30/NCR3 protein is a cell membrane receptor on natural killer (NK) cells that activates cytotoxicity by binding to ligands such as BAG6 and NCR3LG1, stimulating NK cells to respond against neighboring cells (including tumor cells) expressing these ligands. NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived NKp30/NCR3 protein, expressed by HEK293 , with C-His labeled tag. The total length of NKp30/NCR3 Protein, Cynomolgus/Rhesus Macaque (HEK293, His) is 117 a.a., with molecular weight of ~23-32 kDa.

NKp30/NCR3 蛋白作为自然杀伤 (NK) 细胞的细胞膜受体，在结合 BAG6 和 NCR3LG1 等细胞外配体后被激活。配体结合后，NKp30/NCR3 刺激 NK 细胞对表达这些配体的邻近细胞的细胞毒性，从而控制 NK 细胞对肿瘤细胞的细胞毒性。BAG6 与 NCR3 的结合不仅增强了 NK 细胞介导的对未能获得成熟表型的骨髓树突状细胞 (DC) 的杀伤作用，而且还促进了 DC 的成熟。这是通过 NK 细胞释放 TNFA 和 IFNG 来实现的，进一步促进成熟过程。在未配体形式下，NKp30/NCR3 形成同二聚体，并与 CD3Z 以及 NCR3LG1 和 BAG6 相互作用，并通过与 NCR3LG1 和 BAG6 结合而被激活，揭示其在调节免疫反应、NK 细胞毒性和 DC 成熟中的复杂作用。

Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 is immobilized at 0.5 µg/mL (100 µL/well) can bind Recombinant Cynomolgus NKp30/NCR3. The ED₅₀ for this effect is 59.89 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. When Recombinant Human B7-H6 is immobilized at 0.5 µg/mL (100 µL/well) can bind Recombinant Cynomolgus NKp30/NCR3. The ED₅₀ for this effect is 59.89 ng/mL.

Rhesus Macaque

HEK293

C-His

XP_005553604 (L19-G135)

102133932  [NCBI]

LWVSQPPEIRTLEGSSAFLPCSFNASQGRLAIGSVTWFRDEVAPGKEVRNGTPEFRGRLAPLSSSRFLRDHQAELHIWDVRGHDAGIYVCRVEVLGLGVGTGNGTRLVVEKEYPQLG

The protein migrates as an approximately 23-32 kDa band under reducing SDS-PAGE due to glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.