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  3. NT5C3A/NT5C3 蛋白, Human

NT5C3A/NT5C3 蛋白, Human

目录号: HY-P77455
COA 产品使用指南

NT5C3A/NT5C3 蛋白是一种核苷酸酶,优先水解胞苷单磷酸 (CMP) 和 7-甲基鸟苷单磷酸 (m(7)GMP),强调在细胞过程中的潜在作用,并优先水解 CMP。NT5C3A/NT5C3 蛋白, Human 是重组的 NT5C3A/NT5C3 蛋白,由 E. coli 表达,不带标签。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥360 In-stock
10 μg ¥580 In-stock
50 μg ¥1500 In-stock
100 μg ¥2400 In-stock
500 μg ¥7200 询价
1 mg ¥11500 询价
> 1 mg   询价  

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描述

NT5C3A/NT5C3 Protein, a nucleotidase, preferentially hydrolyzes cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP), emphasizing a potential role in cellular processes, with a preference for CMP. NT5C3A/NT5C3 Protein, Human is the recombinant human-derived NT5C3A/NT5C3 protein, expressed by E. coli , with tag free.

研究背景

NT5C3A/NT5C3 蛋白是一种核苷酸酶,对胞苷单磷酸 (CMP) 和 7-甲基鸟苷单磷酸 (m(7)GMP) 表现出特异活性。虽然它对两种底物都表现出活性,但 CMP 似乎是该酶的首选底物。这些催化偏好表明该蛋白质参与特定核苷酸化合物的水解,并可能重点关注细胞过程中的单磷酸胞苷。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9H0P0-1 (M12-L297)

基因 ID
蛋白结构
N-term
NT5C3A (M12-L297)
Accession # Q9H0P0-1
C-term
同用名
Cytosolic 5'-nucleotidase 3A; NT5C3; P5N1; UMPH1
氨基酸序列

MMPEFQKSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYKGKRCPTCHNIIDNCKLVTDECRKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGLLVQQALPKAKLKEIVAESDVMLKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQAGVYHPNVKVVSNFMDFDETGVLKGFKGELIHVFNKHDGALRNTEYFNQLKDNSNIILLGDSQGDLRMADGVANVEHILKIGYLNDRVDELLEKYMDSYDIVLVQDESLEVANSILQKIL

分子量

Approximately 33 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.0, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

NT5C3A/NT5C3 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NT5C3A/NT5C3 Protein, Human
目录号:
HY-P77455
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