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  3. NTAL 蛋白, Human (HEK293, His)

NTAL 蛋白, Human (HEK293, His)

目录号: HY-P71173
产品使用指南

NTAL 蛋白对于肥大细胞中 FCER1 下游信号传导至关重要,并参与 B 细胞中 BCR 介导的信号传导和骨髓细胞中 FCGR1 介导的信号传导。它充当分子桥,在磷酸化时招募 GRB2,将受体激活与细胞内反应联系起来。NTAL 蛋白, Human (HEK293, His) 是重组的 NTAL 蛋白,由 HEK293 表达,带有 C-6*His 标签。NTAL 蛋白, Human (HEK293, His) 全长 217 个氨基酸,分子量为 35-40 kDa。

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描述

NTAL protein is critical for FCER1 downstream signaling in mast cells and is involved in BCR-mediated signaling in B cells and FCGR1-mediated signaling in myeloid cells. It acts as a molecular bridge, recruiting GRB2 upon phosphorylation, linking receptor activation to intracellular responses. NTAL Protein, Human (HEK293, His) is the recombinant human-derived NTAL protein, expressed by HEK293 , with C-6*His labeled tag. The total length of NTAL Protein, Human (HEK293, His) is 217 a.a., with molecular weight of 35-40 kDa.

研究背景

NTAL 蛋白在介导肥大细胞中高亲和力免疫球蛋白 epsilon 受体 (FCER1) 下游信号传导事件中发挥着至关重要的作用。此外,它还参与 B 细胞中 B 细胞抗原受体 (BCR) 介导的信号传导和骨髓细胞中高亲和力免疫球蛋白 γ Fc 受体 I (FCGR1) 介导的信号传导。NTAL 充当分子桥梁,通过在磷酸化时招募 GRB2 来促进受体激活和随后的细胞内反应之间的联系。此外,它表现出与关键信号分子(包括 SOS1、GAB1 和 CBL)的潜在相互作用,强调了它参与协调不同的细胞途径以响应免疫受体激活。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9GZY6 (R27-A243)

基因 ID
蛋白结构
N-term
NTAL (R27-A243)
Accession # Q9GZY6
6*His
C-term
同用名
Linker for Activation of T-Cells Family Member 2; Linker for Activation of B-Cells; Membrane-Associated Adapter Molecule; Non-T-Cell Activation Linker; Williams-Beuren Syndrome Chromosomal Region 15 Protein; Williams-Beuren Syndrome Chromosomal Region 5 Protei
氨基酸序列

RCSRPGAKRSEKIYQQRSLREDQQSFTGSRTYSLVGQAWPGPLADMAPTRKDKLLQFYPSLEDPASSRYQNFSKGSRHGSEEAYIDPIAMEYYNWGRFSKPPEDDDANSYENVLICKQKTTETGAQQEGIGGLCRGDLSLSLALKTGPTSGLCPSASPEEDEESEDYQNSASIHQWRESRKVMGQLQREASPGPVGSPDEEDGEPDYVNGEVAATEA

分子量

35-40 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

NTAL Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
NTAL Protein, Human (HEK293, His)
目录号:
HY-P71173
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