Nucleoprotein/NP 蛋白, HCoV-NL63 (Sf9, His, myc)

Nucleoprotein/NP proteins play a crucial role in assembling viral particles and facilitating the packaging of positive-strand viral genomic RNA into helical ribonucleocapsids (RNPs). Its interaction with the viral genome and membrane protein M is critical for virion assembly. Nucleoprotein/NP Protein, HCoV-NL63 (Sf9, His, myc) is the recombinant Virus-derived Nucleoprotein/NP protein, expressed by Sf9 insect cells , with C-Myc, N-10*His labeled tag. The total length of Nucleoprotein/NP Protein, HCoV-NL63 (Sf9, His, myc) is 377 a.a., with molecular weight of ~ 46.3 kDa.

核蛋白/NP 蛋白在病毒颗粒组装过程中发挥着重要作用，负责将正链病毒基因组 RNA 包装成螺旋核糖核衣壳 (RNP)。它与病毒基因组和膜蛋白 M 之间复杂的相互作用是病毒粒子组装过程的基础。除了其结构作用之外，NP 蛋白还对亚基因组病毒 RNA 转录和整体病毒复制的效率做出了重大贡献。NP 蛋白以单体和寡聚形式存在，形成同源寡聚物并与 RNA 直接相互作用。此外，它与 NSP3 的关联有助于在感染的早期阶段将基因组与新翻译的复制酶-转录酶复合物联系起来，进一步凸显了它在病毒生命周期中的多方面参与。

Virus

Sf9 insect cells

C-Myc;N-10*His

Q6Q1R8 (M1-H377)

2943504  [NCBI]

MASVNWADDRAARKKFPPPSFYMPLLVSSDKAPYRVIPRNLVPIGKGNKDEQIGYWNVQERWRMRRGQRVDLPPKVHFYYLGTGPHKDLKFRQRSDGVVWVAKEGAKTVNTSLGNRKRNQKPLEPKFSIALPPELSVVEFEDRSNNSSRASSRSSTRNNSRDSSRSTSRQQSRTRSDSNQSSSDLVAAVTLALKNLGFDNQSKSPSSSGTSTPKKPNKPLSQPRADKPSQLKKPRWKRVPTREENVIQCFGPRDFNHNMGDSDLVQNGVDAKGFPQLAELIPNQAALFFDSEVSTDEVGDNVQITYTYKMLVAKDNKNLPKFIEQISAFTKPSSIKEMQSQSSHVAQNTVLNASIPESKPLADDDSAIIEIVNEVLH

Approximately 46.3kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.