P4HB 蛋白, Human (HEK293, His)

The P4HB protein is a multifunctional entity that catalyzes disulfide bonding processes at the cell surface and within cells. As a reductase, it modifies outer surface proteins and participates in the formation of disulfide bonds in nascent proteins. P4HB Protein, Human (HEK293, His) is the recombinant human-derived P4HB protein, expressed by HEK293 , with C-6*His labeled tag. The total length of P4HB Protein, Human (HEK293, His) is 488 a.a., with molecular weight of ~58.9 kDa.

P4HB 蛋白是一种多功能实体，可催化二硫键形成、断裂和重排的复杂过程。它位于细胞表面，充当还原酶，裂解与细胞相连的蛋白质的二硫键，并可能导致外表面蛋白质的结构修饰。在细胞内，P4HB 参与新生蛋白质二硫键的形成和重排。当浓度升高时，在 FAM20C 磷酸化的影响下，它充当伴侣，防止错误折叠的蛋白质聚集。相反，在较低浓度下，它通过促进聚集而表现出抗伴侣活性。P4HB 的多功能性延伸到它作为结构亚基参与多种酶，例如脯氨酰 4-羟化酶和微粒体三酰甘油转移蛋白 MTTP。此外，它作为 LGALS9 的受体，通过这种相互作用影响 Th2 T 辅助细胞表面的二硫键还原酶活性，改变质膜的氧化还原状态，并增强细胞迁移。这种多样化的功能强调了 P4HB 在细胞过程中的关键作用，包括蛋白质折叠、氧化还原调节和细胞迁移。

Human

HEK293

C-6*His

P07237 (D18-K505)

5034  [NCBI]

DAPEEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVK

Approximately 58.9 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.