PARP 蛋白, Mouse (sf9, His)

PARP, a poly-ADP-ribosyltransferase, catalyzes poly-ADP-ribosylation, crucial for DNA repair. This protein irreversibly binds to DNA breaks, disrupting repair processes and triggering DNA damage-induced apoptosis. PARP Protein, Mouse (sf9, His) is the recombinant mouse-derived PARP protein, expressed by Sf9 insect cells , with N-His labeled tag.

PARP 蛋白是一种聚 ADP 核糖基转移酶，负责催化蛋白质的聚 ADP 核糖基化，并在 DNA 修复机制中发挥着关键作用。具体来说，这种蛋白质不可逆地与 DNA 断裂结合，阻碍 DNA 修复过程，最终导致 DNA 损伤诱导的细胞凋亡。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

Sf9 insect cells

N-His

Q921K2 (M1-W1014)

11545  [NCBI]

Approximately 115 kDa

Greater than 85% as determined by reducing SDS-PAGE

溶液

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% Glycerol, 0.1 mM TCEP. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.