1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PEPD 蛋白, Human

PEPD 蛋白, Human

目录号: HY-P70992
COA 产品使用指南

PEPD 蛋白是一种二肽酶,可水解羧基末端带有脯氨酸或羟脯氨酸的二肽。由于胶原蛋白中亚氨基酸含量高,因此它在胶原蛋白代谢中发挥着重要作用。PEPD 抑制 p53 对细胞存活和肿瘤生长至关重要。PEPD 蛋白, Human 是重组的 PEPD 蛋白,由 E. coli 表达,不带标签。PEPD 蛋白, Human 全长 492 个氨基酸,分子量为 54-60 kDa。

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规格 价格 是否有货 数量
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5 μg ¥650 In-stock
10 μg ¥1100 In-stock
50 μg ¥3400 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

PEPD Protein, a member of the peptidase family, is a dipeptidase which hydrolyze dipeptides with proline or hydroxyproline at the carboxy terminus. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. PEPD suppression of p53 is essential for cell survival and tumor growth. PEPD Protein, Human is the recombinant human-derived PEPD protein, expressed by E. coli , with tag free. The total length of PEPD Protein, Human is 492 a.a., with molecular weight of 54-60 kDa.

研究背景

PEPD 是肽酶家族的一员,是一种二肽酶,可水解羧基末端带有脯氨酸或羟脯氨酸的二肽。优选的二肽底物是 Gly-Pro,但其他 Xaa-Pro 二肽,例如 Ala-Pro、Met-Pro、Phe-Pro、Val-Pro和 Leu-Pro,可以被 PEPD 裂解。由于胶原蛋白中亚氨基酸含量高,因此它在胶原蛋白代谢中起着重要作用。PEPD 调节干扰素 α/β 受体 IFNAR1 的表达。PEPD 直接与细胞核和细胞质中的 p53 结合,并抑制 p53 的转录依赖性和转录非依赖性活性,这对于细胞存活和肿瘤生长至关重要。此外,PEPD 通过激活 EGFR 下游 PI3K/Akt/mTOR 信号通路刺激成纤维细胞的增殖和迁移,这也刺激 β1-整合素和 IGF-1 受体以及这些受体下游蛋白 (例如FAK、Grb2 和 ERK1/2) 的表达[1][2][3][4]

生物活性

Measured by its ability to cleave a peptide substrate, Gly-Pro-AMC. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 5013.428 pmol/min/µg.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

AAH28295.1 (A2-K493)

基因 ID
蛋白结构
N-term
PEPD (A2-K493)
Accession # AAH28295.1
C-term
同用名
Xaa-Pro dipeptidase; Imidodipeptidase; PeptidaseD; Prolinedipeptidase; PRD; PEPD.
氨基酸序列

AAATGPSFWLGNETLKVPLALFALNRQRLCERLRKNPAVQAGSIVVLQGGEETQRYCTDTGVLFRQESFFHWAFGVTEPGCYGVIDVDTGKSTLFVPRLPASHATWMGKIHSKEHFKEKYAVDDVQYVDEIASVLTSQKPSVLLTLRGVNTDSGSVCREASFDGISKFEVNNTILHPEIVECRVFKTDMELEVLRYTNKISSEAHREVMKAVKVGMKEYELESLFEHYCYSRGGMRHSSYTCICGSGENSAVLHYGHAGAPNDRTIQNGDMCLFDMGGEYYCFASDITCSFPANGKFTADQKAVYEAVLRSSRAVMGAMKPGVWWPDMHRLADRIHLEELAHMGILSGSVDAMVQAHLGAVFMPHGLGHFLGIDVHDVGGYPEGVERIDEPGLRSLRTARHLQPGMVLTVEPGIYFIDHLLDEALADPARASFFNREVLQRFRGFGGVRIEEDVVVTDSGIELLTCVPRTVEEIEACMAGCDKAFTPFSGPK

分子量

54-60 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, 10% Glycerol, pH 8.5 or 50 mM Tris-HCL, 300 mM NaCL, 10% Glycerol, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

PEPD Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
PEPD Protein, Human
目录号:
HY-P70992
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