PFDN1 蛋白, Mouse (His)

The PFDN1 protein is a key player in the immune response, presenting antigen to CD4 T cells by accommodating 10-30 residue peptides in its peptide-binding cleft. PFDN1 plays a role in the endocytic pathway of antigen-presenting cells, specifically in the exogenous antigen presentation pathway, forming heteromers with three MHC class II molecules and a CD74 trimer in the endoplasmic reticulum. PFDN1 Protein, Mouse (His) is the recombinant mouse-derived PFDN1 protein, expressed by E. coli , with N-His labeled tag. The total length of PFDN1 Protein, Mouse (His) is 122 a.a., with molecular weight of ~16 kDa.

HLA-DPB1 是免疫反应中的关键参与者，有助于将抗原衍生的肽呈递给 CD4 T 细胞。HLA-DPB1 的肽结合裂隙在抗原呈递细胞 (APC) 的内吞途径中发挥作用，可容纳一系列 10-30 个残基肽。这种 MHC II 类分子特别参与外源抗原呈递途径，其中来自降解蛋白质的肽呈递在细胞表面上。这一复杂的过程涉及三个 MHC II 类分子与内质网 (ER) 中的 CD74 三聚体的结合，形成异源单体。进入内体/溶酶体系统后，CD74 经历连续降解，最终释放出称为 CLIP（II 类相关不变链肽）的小片段。HLA-DM 可促进 CLIP 的去除，从而稳定 MHC II 类分子，直至高亲和力抗原肽结合。所得的 MHC II 分子-肽复合物随后被转运至细胞膜表面进行识别。值得注意的是，B 细胞和原代树突细胞 (DC) 中的 HLA-DO 调节 HLA-DM 和 MHC II 类分子之间的相互作用。溶酶体微环境以酸化增强为特征，在调节抗原加载到 MHC II 分子、影响蛋白水解和高效肽加载方面发挥着至关重要的作用。HLA-DPB1 的异二聚体结构由 α 和 β 亚基组成，有助于其在抗原呈递中发挥多方面的作用。

Mouse

E. coli

N-6*His

Q9CQF7 (M1-Q122)

67199  [NCBI]

MAASVDLELKKAFTELQAKVIDTQQKVKLADIQIEQLNRTKKHAHLTDTEIMTLVDETNMYEGVGRMFILQSKEVIHNQLLEKQKIAEEKIKELEQKKSYLERSVKEAEDNIREMLMARRAQ

Approximately 16 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.