PFDN2 蛋白, Human (His)

The PFDN2 protein selectively binds to the cytoplasmic chaperone protein (c-CPN) and directs the protein for targeted transfer. It also interacts with nascent peptides and actively promotes correct folding in complex cellular environments. PFDN2 Protein, Human (His) is the recombinant human-derived PFDN2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PFDN2 Protein, Human (His) is 154 a.a., with molecular weight of ~19 kDa.

PFDN2 蛋白与胞质伴侣蛋白 (c-CPN) 表现出特异性结合亲和力，促进蛋白质靶向转移至该伴侣。此外，PFDN2 与新生多肽链相互作用，在复杂的细胞环境中积极促进其正确折叠，并具有许多非天然蛋白质的竞争途径。作为异六聚体，PFDN2包含两个PFD-α型亚基和四个PFD-β型亚基。它作为 PAQosome 复合体的一部分发挥着至关重要的作用，与其他重要组件协作，例如 R2TP 复合体成员（RUVBL1、RUVBL2、RPAP3 和 PIH1D1）和 URI 复合体成员（PFDN6、PDRG1、UXT 和 URI1）以及ASDURF、POLR2E 和 DNAAF10/WDR92。该复合物是各种蛋白质复合物的生物发生不可或缺的一部分。值得注意的是，PFDN2 与 URI1 进行磷酸化依赖性相互作用，表现出生长依赖性相互作用方式。

Human

E. coli

N-6*His

Q9UHV9 (M1-S154)

5202  [NCBI]

MAENSGRAGKSSGSGAGKGAVSAEQVIAGFNRLRQEQRGLASKAAELEMELNEHSLVIDTLKEVDETRKCYRMVGGVLVERTVKEVLPALENNKEQIQKIIETLTQQLQAKGKELNEFREKHNIRLMGEDEKPAAKENSEGAGAKASSAGVLVS

Approximately 19 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.