Progranulin/PGRN 蛋白, Human (HEK293, C-His)

EpCAM/TROP1 protein is a cell surface protein expressed in a variety of epithelial tissues. It plays a crucial role in cell adhesion, proliferation and differentiation. Progranulin/PGRN Protein, Human (HEK293, C-His) is the recombinant human-derived Progranulin/PGRN protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Progranulin/PGRN Protein, Human (HEK293, C-His) is 576 a.a., with molecular weight of 80-95 kDa.

颗粒体蛋白前体/PGRN 蛋白代表一种 88 kDa 的前体蛋白，也称为上皮蛋白原和 PC 细胞衍生的生长因子，从该蛋白中裂解出称为颗粒体蛋白的分泌型糖基化肽家族。这种裂解产生成熟的颗粒蛋白，进一步加工成活性 6 kDa 肽，包括颗粒蛋白 A、颗粒蛋白 B、颗粒蛋白 C 等。上皮蛋白 1 和 2 分别与颗粒蛋白 A 和 B 同义。颗粒蛋白家族具有 7.5 个高度保守的 12-半胱氨酸颗粒蛋白/上皮蛋白基序重复，在调节细胞生长中发挥着至关重要的作用，其各种成员充当抑制剂、刺激剂或对细胞生长具有双重作用。颗粒体蛋白前体/PGRN 广泛表达，在骨髓 (RPKM 141.7)、肺 (RPKM 139.8) 和 25 个其他组织中表现出升高的水平，强调了其在多个器官的不同生理过程中的潜在重要性。

1.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.6157 μg/mL, corresponding to a specific activity is 1.62×10³ U/mg.
2.Measured by its ability to inhibit THP-1 human acute monocyte migration in the presence of 10 ng/mL MCP-1.

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.6157 μg/mL, corresponding to a specific activity is 1.62×10³ U/mg.

Human

HEK293

C-10*His

NP_002078.1/P28799 (T18-L593)

2896  [NCBI]

TRCPDGQFCPVACCLDPGGASYSCCRPLLDKWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTCVAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRWDAPLRDPALRQLL

Approximately 80-95 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.