RHEB 蛋白, Human (sf9, N-His)

RHEB is an important small GTPase that acts as an allosteric activator of the mTORC1 complex, a key nutrient sensor that controls cell growth and biomass production. In response to signals such as nutrients and growth factors, RHEB activates the MTOR kinase within mTORC1 by inducing conformational changes. RHEB Protein, Human (sf9, N-His) is the recombinant human-derived RHEB protein, expressed by P. pastoris , with N-His labeled tag. The total length of RHEB Protein, Human (sf9, N-His) is 181 a.a., with molecular weight of ~21 kDa.

RHEB 是一种小型 GTP 酶，作为经典 mTORC1 复合体的关键变构激活剂，mTORC1 复合体是协调细胞生物量生成和生长的关键营养传感器。为了响应营养物质、生长因子或氨基酸等线索，RHEB 在激活 mTORC1 复合物内 MTOR 的蛋白激酶活性方面发挥着特定作用。这种激活是通过诱导构象变化来实现的，该构象变化会对齐 MTOR 活性位点中的残基，从而增强关键下游效应子的磷酸化，如核糖体蛋白 S6 激酶（RPS6KB1 和 RPS6KB2）和 EIF4EBP1 (4E-BP1)。此外，RHEB 对于 TSC-TBC 复合物定位到溶酶体膜至关重要，为 mTORC1 活性提供空间调节。相反，在饥饿期间，TSC-TBC 复合物会使 RHEB 失活，从而阻止 mTORC1 激活并有助于细胞在营养缺乏条件下的适应。值得注意的是，RHEB 表现出较低的内在 GTPase 活性，进一步强调了其在动态调节 mTORC1 信号传导中的作用。

Human

P. pastoris

N-His

Q15382 (M1-C181)

6009  [NCBI]

Approximately 21 kDa

Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, 10% Glycerol, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.