SAA1 蛋白, Human (His)

SAA1 Protein, a major acute phase protein, forms a homohexamer comprising dimers of trimers. While the native form doesn't spontaneously form amyloid fibrils, partial proteolysis can induce amyloid fibril generation. SAA1 also serves as an apolipoprotein in the HDL complex and shows affinity for heparin. SAA1 Protein, Human (His) is the recombinant human-derived SAA1 protein, expressed by E. coli , with N-6*His labeled tag.

SAA1蛋白是一种主要的急性期蛋白，以由二聚体或三聚体组成的同源六聚体存在。虽然天然的、未变性的形式不会自发形成淀粉样蛋白原纤维，但部分蛋白水解可导致淀粉样蛋白原纤维的生成。此外，SAA1 在 HDL 复合物中充当载脂蛋白，并表现出对肝素的亲和力。

Measured by its ability to chemoattract THP-1 cells. The ED₅₀ for this effect is 70.88 ng/mL, corresponding to a specific activity is 1.411×10⁴ U/mg.

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  Measured by its ability to chemoattract THP-1 cells. The ED₅₀ for this effect is 70.88 ng/mL, corresponding to a specific activity is 1.411×10⁴ U/mg.

Human

E. coli

N-6*His

AAH07022.1 (R19-Y122)

6288  [NCBI]

RSFFSFLGEAFDGARDMWRAYSDMREANYIGSDKYFHARGNYDAAKRGPGGVWAAEAISDARENIQRFFGHGAEDSLADQAANEWGRSGKDPNHFRPAGLPEKY

Approximately 13.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl,1 mM EDTA, pH 8.0 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.