2. 重组蛋白
  3. Viral Proteins
  4. SARS-CoV-2 Proteins
  5. SARS-CoV-2 3C-like Protease
  6. SARS-CoV-2 3CLpro/3C-like protease 蛋白

SARS-CoV-2 3CLpro/3C-like protease 蛋白

目录号: HY-P78275
COA 产品使用指南

SARS-CoV-2 3CLpro/3C-like protease 蛋白 是重组的 SARS-CoV-2 3CLpro/3C 样蛋白酶蛋白，由 E. coli 表达，不带标签。SARS-CoV-2 3CLpro/3C-like protease 蛋白 全长 306 个氨基酸，分子量约为 ~33.8 kDa。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ￥950 In-stock
10 μg ￥1550 In-stock
20 μg ￥2350 In-stock
50 μg ￥4280 In-stock
100 μg   询价  

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  • 生物活性

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描述

SARS-CoV-2 3CLpro/3C-like protease Protein is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by E. coli , with tag free. The total length of SARS-CoV-2 3CLpro/3C-like protease Protein is 306 a.a., with molecular weight of ~33.8 kDa.
生物活性

Measured by its ability to cleave a fluorogenic peptide substrate Dabcyl-KTSAVLQSGFRKME-Edans and the specific activity is ≥1068.3 nmol/min/mg.
种属

Virus
表达系统

E. coli
标签

Tag Free
蛋白编号

YP_009725301.1 (S1-Q306)
基因 ID

43740578  [NCBI]
蛋白结构
N-term
SARS-CoV-2 3CLpro (S1-Q306)
Accession # YP_009725301.1
C-term
同用名
3C-like protease; 3CL Protease; 3CL-Mpro; 3CL Pro; COVID-19; M Proteinase; Mpro
分子量

Approximately 33.8 kDa
纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

溶液
组分

Supplied as a 0.22 μm filtered solution of 25 mM HEPES, 2.5 mM DTT, 10% glycerol, pH 7.5.

内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

N/A.
保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
运输条件

Shipping with dry ice.
文件资料

COA (208 KB)

产品使用指南 (1213 KB)

SARS-CoV-2 3CLpro/3C-like protease Protein 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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