SARS-CoV-2 RDRP 蛋白 (sf9, His)

The function of viral RNA transcription and replication is performed by a specific RNA dependent RNA polymerase (RdRp) region present in ORF1ab, including non-structure proteins NSP12 with nucleotidyltransferase activity and NSP13 with a Zinc-binding domain involved in replication and transcription. The catalytic subunit (Nsp12) RdRp enzyme is the core component of multisubunit replication and transcription complex of NSPs. SARS-CoV-2 RDRP Protein (sf9, His) is the recombinant Virus-derived SARS-CoV-2 RDRP protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SARS-CoV-2 RDRP Protein (sf9, His) is 932 a.a., with molecular weight of ~91.3 kDa.

病毒 RNA 转录和复制的功能是由 ORF1ab 中存在的特定 RNA 依赖 RNA 聚合酶 (RdRp) 区域完成的，包括具有核苷酸转移酶活性的非结构蛋白 NSP12 和具有参与复制和转录的锌结合结构域的非结构蛋白 NSP13。NSPs 的多亚基复制和转录复合体决定了 SARS-CoV-2 的复制，催化亚基 (Nsp12) RdRp 酶是该复合体的核心成分。Nsp12 本身几乎没有活性，需要额外的因子 (Nsp7 和 Nsp8) 才能正常发挥作用^[1][2]。

Virus

Sf9 insect cells

C-His

YP_009725307 (S1-Q932)

43740578  [NCBI]

Approximately 91.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 300 mM NaCl, 10% Glycerol, 22.5% Trehalose, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.