SHH 蛋白, Mouse (HEK293, His)

Sonic hedgehog (SHH) undergoes autoproteolysis in the endoplasmic reticulum to produce ShhN and ShhC.ShhN is covalently linked to cholesterol to form ShhNp, a morphogen critical for developmental patterning.SHH Protein, Mouse (HEK293, His) is the recombinant mouse-derived SHH protein, expressed by HEK293 , with C-His labeled tag.

声波刺猬蛋白 (SHH) 前体经历自蛋白水解和胆固醇转移酶活性，导致蛋白质裂解成两部分：ShhN 和 ShhC。然后新生成的 ShhN 在其 C 末端与胆固醇部分共价连接。这个过程发生在内质网中。ShhC 随后在内质网中被降解。脂化的 ShhNp 作为形态发生素，在各种发育模式事件中发挥着至关重要的作用。它诱导神经管和体节的腹侧细胞命运，参与肢芽的前后轴模式，并且对于轴突引导至关重要。SHH 与 patched (PTCH1) 受体结合，该受体与 smoothened (SMO) 结合，激活靶基因的转录。在缺乏 SHH 的情况下，PTCH1 抑制 SMO 的组成型信号活动。

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 3.6-40 μg/mL.

Mouse

HEK293

C-His

Q62226 (C25-G198)

20423  [NCBI]

Approximately 23 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.