SKP2 蛋白, Human (His-SUMO)

SKP2 serves as a substrate recognition component in the SCF E3 ubiquitin-protein ligase complex, directing ubiquitination and proteasomal degradation of key proteins in the cell cycle, signaling, and transcription. It crucially recognizes phosphorylated CDKN1B/p27kip and regulates the G1/S transition. SKP2 Protein, Human (His-SUMO) is the recombinant human-derived SKP2 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of SKP2 Protein, Human (His-SUMO) is 424 a.a., with molecular weight of ~63.8 kDa.

SKP2 作为 SCF（SKP1-CUL1-F-box 蛋白）E3 泛素-蛋白连接酶复合物内的底物识别组件，协调在细胞周期进程、信号转导和转录中至关重要的靶蛋白的泛素化和随后的蛋白酶体降解。它特异性识别磷酸化的 CDKN1B/p27kip，在 G1/S 转换的调节中发挥着至关重要的作用。SKP2 的底物谱涵盖 ORC1、CDT1、RBL2、KMT2A/MLL1、CDK9、RAG2、FOXO1、UBP43、YTHDF2、MYC、TOB1 和 TAL1 等蛋白质。此外，SKP2 参与泛素介导的丙型肝炎病毒非结构蛋白 5A 的蛋白酶体降解，显示出针对该病毒的抗病毒活性。SKP2 相互作用的复杂性质强调了其通过靶向蛋白质降解来控制多种细胞过程的重要性。

Human

E. coli

N-SUMO;N-6*His

Q13309 (M1-L424)

6502  [NCBI]

MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKEEPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPDELLLGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGVIAFRCPRSFMDQPLAEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRLSDPIVNTLAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQVAVAHVSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQLNYLQHLSLSRCYDIIPETLLELGEIPTLKTLQVFGIVPDGTLQLLKEALPHLQINCSHFTTIARPTIGNKKNQEIWGIKCRLTLQKPSCL

Approximately 63.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.