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  4. SLC30A8 蛋白, Human (Sf9, His, MBP, FLAG)

SLC30A8 蛋白, Human (Sf9, His, MBP, FLAG)

目录号: HY-P702032
产品使用指南

SLC30A8 蛋白是一种质子偶联的锌离子反向转运蛋白，可协调必需的锌进入胰腺 β 细胞颗粒并显着调节胰岛素分泌。SLC30A8 蛋白, Human (Sf9, His, MBP, FLAG) 是重组的 SLC30A8 蛋白，由 Sf9 insect cells 表达，带有 N-MBP, C-Flag, N-8*His 标签。SLC30A8 蛋白, Human (Sf9, His, MBP, FLAG) 全长 368 个氨基酸。

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描述

The SLC30A8 protein is a proton-coupled zinc ion antiporter that coordinates essential zinc entry into pancreatic beta cell granules and significantly regulates insulin secretion. SLC30A8 Protein, Human (Sf9, His, MBP, FLAG) is the recombinant human-derived SLC30A8 protein, expressed by Sf9 insect cells , with N-MBP, C-Flag, N-8*His labeled tag. The total length of SLC30A8 Protein, Human (Sf9, His, MBP, FLAG) is 368 a.a., .
研究背景

SLC30A8 蛋白充当质子偶联的锌离子反向转运蛋白，协调锌进入胰腺 β 细胞分泌颗粒内腔的关键过程。这一关键功能在胰岛素分泌的调节中发挥着重要作用。通过积极参与分泌颗粒内锌离子的运输，SLC30A8 有助于控制胰腺 β 细胞释放胰岛素的复杂过程。该蛋白质在介导锌的质子耦合转运方面的独特作用强调了其在维持响应生理刺激的适当胰岛素调节所需的微妙平衡方面的重要性。
种属

Human
表达系统

Sf9 insect cells
标签

N-MBP;C-Flag;N-8*His
蛋白编号

Q8IWU4 (E2-D369)
基因 ID

169026
蛋白结构
N-term
8*His-MBP
SLC30A8 (E2-D369)
Accession # Q8IWU4
Flag
C-term
同用名
SLC30A8; Zinc transporter 8; ZnT-8; Solute carrier family 30 member 8
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

SLC30A8 Protein, Human (Sf9, His, MBP, FLAG) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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