SMYD2 蛋白, Human (sf9, His)

The SMYD2 protein is a multifunctional methyltransferase that can modify histones and non-histone proteins, including p53/TP53 and RB1. Notably, it trimethylates H3 at "Lys-4" (H3K4me3), which is critical for gene activation. SMYD2 Protein, Human (sf9, His) is the recombinant human-derived SMYD2 protein, expressed by Sf9 insect cells , with N-His labeled tag.

SMYD2 蛋白是一种多功能蛋白赖氨酸 N-甲基转移酶，具有甲基化组蛋白和非组蛋白（例如 p53/TP53 和 RB1）的能力。值得注意的是，它在体内特异性地将组蛋白 H3 的“Lys-4”(H3K4me3) 三甲基化，这是与基因激活相关的重要修饰。SMYD2 的酶活性需要与 HSP90alpha 相互作用，进一步凸显了其对细胞调节网络的功能依赖。引人注目的是，SMYD2 在 p53/TP53 上表现出较高的甲基转移酶活性，它会将“Lys-370”单甲基化，从而导致 DNA 结合活性和随后的转录调控降低。此外，SMYD2 通过单甲基化“Lys-860”在 RB1 的表观遗传修饰中发挥作用，显示出其对组蛋白和非组蛋白的广泛影响，从而有助于细胞环境内复杂的表观遗传调控。

Human

Sf9 insect cells

N-10*His

Q9NRG4-1 (M1-H433)

56950  [NCBI]

MRAEGLGGLERFCSPGKGRGLRALQPFQVGDLLFSCPAYAYVLTVNERGNHCEYCFTRKEGLSKCGRCKQAFYCNVECQKEDWPMHKLECSPMVVFGENWNPSETVRLTARILAKQKIHPERTPSEKLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHLGFPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLYPTEDRNDRLRDSYFFTCECQECTTKDKDKAKVEIRKLSDPPKAEAIRDMVRYARNVIEEFRRAKHYKSPSELLEICELSQEKMSSVFEDSNVYMLHMMYQAMGVCLYMQDWEGALQYGQKIIKPYSKHYPLYSLNVASMWLKLGRLYMGLEHKAAGEKALKKAIAIMEVAHGKDHPYISEIKQEIESH

Approximately 50 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 10% Glycerol, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.