TIE-2 蛋白, Rhesus Macaque (HEK293, His)

TIE-2 Protein, a tyrosine-protein kinase, acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4, exerting comprehensive control over angiogenesis, endothelial cell behavior, and vascular stability. It regulates diverse processes, including endothelial cell survival, proliferation, migration, adhesion, and actin cytoskeleton reorganization, while also playing a crucial role in maintaining vascular quiescence and preventing the leakage of pro-inflammatory plasma proteins and leukocytes from blood vessels. TIE-2 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived TIE-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of TIE-2 Protein, Rhesus Macaque (HEK293, His) is 723 a.a., with molecular weight of ~84.2 KDa.

TIE-2 是一种酪氨酸蛋白激酶，作为 ANGPT1、ANGPT2 和 ANGPT4 的细胞表面受体，对血管生成、内皮细胞行为和血管稳定性发挥全面控制作用。它调节多种过程，包括内皮细胞存活、增殖、迁移、粘附和肌动蛋白细胞骨架重组，同时在维持血管静止和防止促炎血浆蛋白和白细胞从血管中渗漏方面发挥着至关重要的作用，从而产生抗炎作用。TIE-2 对于胚胎发育和出生后造血过程中的正常血管生成至关重要，在出生后表现出背景依赖性血管生成激活或抑制。在静止血管中，ANGPT1 寡聚化，将 TIE-2 募集至细胞间接触，激活磷脂酰肌醇 3-激酶和 AKT1 信号级联，促进血管稳定性。相反，在缺乏细胞间粘附的迁移内皮细胞中，ANGPT1 将 TIE-2 招募到细胞外基质接触处，通过粘着斑复合物的形成和下游激酶的激活刺激萌芽血管生成。ANGPT1 信号传导诱导受体二聚化和自磷酸化，为支架蛋白和效应物提供结合位点。ANGPT2 的调节、TIE1 异二聚体的形成以及可溶性胞外结构域的蛋白水解加工有助于 TIE-2 信号传导的复杂调节，其中可溶性结构域充当血管生成素的诱饵受体。TIE-2 磷酸化 DOK2、GRB7、GRB14、PIK3R1、SHC1 和 TIE1^[1][2][3][4]。

Immobilized Rhesus Macaque TIE-2 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Angiopoietin-2. The ED₅₀ for this effect is 11.97 ng/mL.

Rhesus Macaque

HEK293

C-His

XP_001105270 (A23-K745)

707455  [NCBI]

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNRLCTVCVNNGVCHEDTGECICPPGFMGRTCEKACEQHTFGRTCKERCSGQDGCKSYVFCLPDPYGCSCATGWKGLQCNEACHHGFYGPDCKLRCSCSNGETCDRFQGCLCSPGRQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVWVCSANTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWRHIQVTNEIVTLNHLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESEAPADLGGGK

Approximately 115 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.