TRAIL R1/TNFRSF10A 蛋白, Human (Biotinylated, HEK293, His-Avi)

TRAIL R1/TNFRSF10A protein is a receptor for TNFSF10/TRAIL, which recruits caspase-8 through FADD to initiate cell apoptosis and form a death-inducing signaling complex (DISC). It activates NF-kappa-B and interacts with TRADD and RIPK1 in the monomeric state. TRAIL R1/TNFRSF10A Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived TRAIL R1/TNFRSF10A protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of TRAIL R1/TNFRSF10A Protein, Human (Biotinylated, HEK293, His-Avi) is 206 a.a., with molecular weight of 25-30 kDa.

TRAIL R1/TNFRSF10A 蛋白充当细胞毒性配体 TNFSF10/TRAIL 的受体。激活后，接头分子 FADD 将 caspase-8 募集至受体，形成死亡诱导信号复合物 (DISC)，导致 caspase-8 蛋白水解激活并启动随后的 caspase 级联，介导细胞凋亡。此外，TRAIL R1/TNFRSF10A 还可促进 NF-kappa-B 的激活。在单体状态下，它可以与 TRADD 和 RIPK1 相互作用。此外，TRAIL R1/TNFRSF10A与TNFRSF10B形成同源寡聚物和异源寡聚物，并且三个TRAIL R1分子与TNFSF10同源三聚体相互作用。该受体还与 ARAP1 和 ZDHHC3 相互作用，进一步凸显其参与复杂的信号网络。在没有刺激的情况下，TRAIL R1/TNFRSF10A 与 BIRC2、DDX3X 和 GSK3B 相互作用，并且这种相互作用在受体刺激时增强，并伴随着 DDX3X 和 BIRC2 的裂解。这些复杂的相互作用强调了 TRAIL R1/TNFRSF10A 在细胞凋亡和信号传导途径中的多方面作用。

Immobilized Human TRAIL,No Tag at 5ug/ml (100ul/well) on the plate.Dose response curve for Biotinylated Human TRAIL R1,His Tag with the EC₅₀ of 0.32 ug/ml determined by ELISA.

Human

HEK293

C-Avi;C-His

O00220 (P34-N239)

8797  [NCBI]

25-30 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.