TWSG1 蛋白, Mouse (HEK293, His)

The TWSG1 protein may contribute to the formation of the dorsal-ventral axis by counteracting BMP signaling and forming a ternary complex with CHRD and BMP to prevent BMP from binding to the receptor. Notably, TWSG1 exhibits anti-BMP and pro-BMP activities. TWSG1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TWSG1 protein, expressed by HEK293 , with C-His labeled tag. The total length of TWSG1 Protein, Mouse (HEK293, His) is 198 a.a., with molecular weight of ~34 kDa.

TWSG1 蛋白可能参与背腹轴的形成。它似乎通过与 CHRD 和 BMP 形成三元复合物来抵消 BMP 信号传导，从而阻止 BMP 与其受体结合来发挥其功能。有趣的是，TWSG1 表现出抗 BMP 和亲 BMP 活性。它通过裂解和降解 CHRD 来实现后者，从而导致从三元复合物中释放 BMP。这种双重作用表明 TWSG1 可能是 BMP 介导过程的关键调节因子，特别是在软骨发育和软骨细胞分化中。此外，它还可能在胸腺细胞发育中发挥作用。TWSG1 与 CHRD 和/或 BMP4 相互作用，增强 CHRD/BMP4 复合物的形成，并且还与 BMP7 相互作用。需要进一步研究以充分理解 TWSG1 运作的精确机制和信号通路。

Measured by its ability to inhibit BMP-6-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 0.8289 µg/mL in the presence of 150 ng/mL of Recombinant Human BMP-6, corresponding to a specific activity is 1.206×10³ U/mg.

Mouse

HEK293

C-His

Q9EP52 (C25-F222)

65960  [NCBI]

CNKALCASDVSKCLIQELCQCRPGEGNCPCCKECMLCLGALWDECCDCVGMCNPRNYSDTPPTSKSTVEELHEPIPSLFRALTEGDTQLNWNIVSFPVAEELSHHENLVSFLETVNQLHHQNVSVPSNNVHAPFPSDKERMCTVVYFDDCMSIHQCKISCESMGASKYRWFHNACCECIGPECIDYGSKTVKCMNCMF

Approximately 27-33 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.