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  3. VSNL1 蛋白, Human

VSNL1 蛋白, Human

目录号: HY-P76694
产品使用指南

VSNL1 在体外介导视紫红质磷酸化抑制,其活性取决于钙水平。这意味着 VSNL1 在调节视紫红质磷酸化动力学中发挥作用,这对于感光细胞信号传导至关重要。VSNL1 蛋白, Human 是重组的 VSNL1 蛋白,由 E. coli 表达,不带标签。VSNL1 蛋白, Human 全长 190 个氨基酸,分子量约为 ~19 kDa。

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描述

VSNL1 mediates rhodopsin phosphorylation inhibition in vitro and its activity is dependent on calcium levels. This implies that VSNL1 plays a role in regulating rhodopsin phosphorylation dynamics, which is critical for photoreceptor cell signaling. VSNL1 Protein, Human is the recombinant human-derived VSNL1 protein, expressed by E. coli , with tag free. The total length of VSNL1 Protein, Human is 190 a.a., with molecular weight of ~19 kDa.

研究背景

VSNL1 蛋白在体外充当视紫红质磷酸化抑制的调节剂,并且这种调节依赖于钙水平。这表明 VSNL1 在调节视紫红质的磷酸化动力学中发挥作用,视紫红质是感光细胞信号级联中的关键过程。VSNL1 活性的钙依赖性意味着它对细胞内钙浓度的反应,可能将其功能与细胞信号事件或参与视觉过程调节的钙介导途径联系起来。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P62760 (G2-K191)

基因 ID
蛋白结构
N-term
VSNL1 (G2-K191)
Accession # P62760
C-term
同用名
Visinin-like protein 1; VILIP; VLP-1; Hippocalcin-like protein 3; HLP3; VSNL1; VISL1
分子量

Approximately 19 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

VSNL1 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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