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  3. VSIG8 蛋白, Mouse (HEK293, C-His)

VSIG8 蛋白, Mouse (HEK293, C-His)

目录号: HY-P72427A
COA 产品使用指南

VSIG8 蛋白, Mouse (HEK293, C-His) 是重组的 VSIG8 蛋白,由 HEK293 表达,带有 C-6*His 标签。VSIG8 蛋白, Mouse (HEK293, C-His) 全长 241 个氨基酸,分子量约为 ~27 kDa。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥900 In-stock
10 μg ¥1500 In-stock
50 μg ¥4200 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

VSIG8 Protein, Mouse (HEK293, C-His) is the recombinant mouse-derived VSIG8 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of VSIG8 Protein, Mouse (HEK293, C-His) is 241 a.a., with molecular weight of ~27 kDa.

生物活性

Measured by its ability to chemoattract Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.7887 μg/ml, corresponding to a specific activity is 1267.91 units/mg.

  • Measured by its ability to chemoattract Jurkat human T-lymphocyte leukemia cells. The ED50 this effect is 0.7887 μg/mL, corresponding to a specific activity is 1267.91 units/mg.
种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q6P3A4-1 (V22-G262)

基因 ID
蛋白结构
N-term
VSIG8 (V22-G262)
Accession # Q6P3A4-1
6*His
C-term
同用名
V-set and immunoglobulin domain-containing protein 8; Vsig8
氨基酸序列

VRINGDGQEVMYLAEGDNVRLGCPYLLDPEDLGTNSLDIEWMQVNSEPSHRENVFLTYQDKRIGHGNLPHLQQRVRFAASDPSQYDASINLMNLQVSDTATYECRVKKTTMATRKVIVTVQARPAVPMCWTEGHMSKGNDVVLKCFANGGSQPLSYKWAKISGHSHPYRAGAYHSQHSFHSELSYQESFHSTINQGLGNGDLLLKGINADDDGLYQCTVANHVGYSVCVVEVKVSDSQRVG

分子量

Approximately 27 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

VSIG8 Protein, Mouse (HEK293, C-His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
VSIG8 Protein, Mouse (HEK293, C-His)
目录号:
HY-P72427A
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