WARS 蛋白, Human (His)

WARS includes isomer 1, isomer 2, T1-TrpRS and T2-TrpRS, and has aminoacylation activity, except T2-TrpRS. Unlike isoform 1, isoform 2, T1-TrpRS and T2-TrpRS, exhibit vasostatic activity. WARS Protein, Human (His) is the recombinant human-derived WARS protein, expressed by E. coli , with N-6*His labeled tag. The total length of WARS Protein, Human (His) is 471 a.a., with molecular weight of ~60.0 kDa.

WARS 包括异构体 1、异构体 2、T1-TrpRS 和 T2-TrpRS，显示出氨酰化活性，但 T2-TrpRS 是例外，因为它缺乏这种酶促功能。就血管抑制活性而言，Isoform 2、T1-TrpRS和T2-TrpRS表现出这种特性，而Isoform 1不具有血管抑制能力。特别是，T2-TrpRS 通过抑制内皮细胞中流体剪切应力激活的反应而脱颖而出。WARS 在调节关键信号通路中发挥着关键作用，包括 ERK、Akt 和 eNOS 激活，这些信号通路与血管生成、细胞骨架重组和响应剪切应力的基因表达相关。这种多方面的功能强调了 WARS 参与复杂的细胞过程及其对血管反应的潜在影响。

Human

E. coli

N-6*His

P23381 (M1-Q471)

7453  [NCBI]

MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ

Approximately 60.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 8% Trehalose, 4% Mannitol, 50 mM NaCl, 0.05% Tween 80, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.