PSI-7409 tetrasodium

目录号: HY-15745A 纯度: 99.31%: FAQs
Data Sheet SDS COA 产品使用指南

摩尔计算器

PSI-7409 tetrasodium 是一种 sofosbuvir (PSI-7977) 的 5'-三磷酸活性代谢物，能够抑制 HCV NS5B polymerases，对 GT 1b_Con1，GT 2a_JFH1，GT 3a 和 GT 4a NS5B 聚合酶的 IC₅₀ 值分别为 1.6，2.8，0.7 和 2.6 μM。

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PSI-7409 tetrasodium Chemical Structure

CAS No. : 1621884-22-7

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规格	价格	是否有货
1 mg	￥2600	In-stock
5 mg	现货	询价
10 mg		询价
50 mg		询价

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Customer Review

Other Forms of PSI-7409 tetrasodium:

PSI-7409 询价

MCE 顾客使用本产品发表的 6 篇科研文献

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

PSI-7409 tetrasodium is an active 5'-triphosphate metabolite of sofosbuvir (PSI-7977), inhibiting HCV NS5B polymerases, with IC₅₀s of 1.6, 2.8, 0.7 and 2.6 μM for GT 1b_Con1, GT 2a_JFH1, GT 3a, and GT 4a NS5B polymerases, respectively.

IC₅₀ & Target

IC50: 1.6 μM (GT 1b_Con1), 2.8 μM (GT 2a_JFH1), 0.7 μM (GT 3a), 2.6 μM (GT 4a)^[1]

体外研究
(In Vitro)

PSI-7409 tetrasodium 是一种活性 5'-三磷酸代谢物，可抑制 HCV NS5B 聚合酶，对 GT 1b_Con1、GT 2a_JFH1、GT 3a 和 GT 的 IC₅₀ 分别为 1.6、2.8、0.7 和 2.6 μM分别为 4a NS5B 聚合酶。PSI-7409 也弱抑制人类 DNA 聚合酶 α，IC₅₀ 为 550 μM，但对 DNA Pol β 和 γ 没有抑制作用^[1]。在克隆 A 细胞中，PSI-7409 的水平在 48 小时内逐渐增加至约 25 μM 的最大浓度。PSI-7409 在原代人肝细胞中以更快的速度形成，在 4 小时时达到 ~100 μM 的最大细胞内浓度，并在该浓度下保持 48 小时^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

588.09

Formula

C₁₀H₁₂FN₂Na₄O₁₄P₃

CAS 号

1621884-22-7

性状

固体

颜色

White to off-white

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

细胞实验：

H₂O 中的溶解度 : ≥ 100 mg/mL (170.04 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7004 mL	8.5021 mL	17.0042 mL
5 mM	0.3401 mL	1.7004 mL	3.4008 mL
10 mM	0.1700 mL	0.8502 mL	1.7004 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (170.04 mM); 澄清溶液; 超声助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: 99.31%

选择批次：

Data Sheet (622 KB) SDS (251 KB)

COA (196 KB) HNMR (515 KB) CNMR (1169 KB) RP-HPLC (258 KB) MS (70 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Lam AM, et al. PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96. [Content Brief]

[2]. Murakami E, et al. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. J Biol Chem. 2010 Nov 5;285(45):34337-47. [Content Brief]

Kinase Assay ^[1]	Human DNA polymerase α, β, or γ is assayed in a 10-μL reaction mixture containing 50 mM Tris (pH 7.5), 50 mM NaCl, 3 mU/μL activated calf thymus DNA, a 20 μM concentration of all four natural deoxynucleoside triphosphates, 4 μCi [α-³²P]dCTP, 5 mM MgCl₂, and increasing concentrations of PSI-7409 (up to 1 mM), D-ddFCTP, or aphidicolin. DNA polymerase α, β, or γ is added to the reaction mixture to give final concentrations of 20, 18, and, 50 μg/mL, respectively. All reactions are run at 37°C and quenched at 30 min by mixing with 1 μL of 0.5 M EDTA. The radiolabeled products are quantified. A nonlinear fit is performed to determine the IC₅₀. The activity of RNA polymerase II is determined in a 25-μL in vitro transcription reaction mixture containing 100 ng of cytomegalovirus (CMV) immediate-early promoter DNA, 400 μM ATP, CTP, and UTP, 16 μM GTP, 10 μCi [α-³²P]GTP, 3 mM MgCl₂, and various concentrations of PSI-7409 (up to 1 mM), 3'-dCTP, or α-amanitin in transcription buffer (20 mM HEPES [pH 7.9], 100 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, and 20% glycerol). All reactions are run at 30°C and quenched at 60 min by mixing with 125 μL of stop solution (0.3 M Tris-HCl [pH 7.4], 0.3 M sodium acetate, 0.5% SDS, 2 mM EDTA, and 3 μg/mL tRNA). The RNA product is purified. The resulting samples contains 12 μL and the same volume of gel loading dye (98% formamide, 10 mM EDTA, 0.1% xylene cyanol, and 0.1% bromophenol blue) is added. The samples are heated at 90°C for 5 min and loaded onto a 6% polyacrylamide sequencing gel. After running, the gel is exposed to a phosphorscreen, and the product is visualized and quantified by using a phosphorimager^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Lam AM, et al. PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96. [Content Brief] [2]. Murakami E, et al. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. J Biol Chem. 2010 Nov 5;285(45):34337-47. [Content Brief]

Kinase Assay
^[1]

Human DNA polymerase α, β, or γ is assayed in a 10-μL reaction mixture containing 50 mM Tris (pH 7.5), 50 mM NaCl, 3 mU/μL activated calf thymus DNA, a 20 μM concentration of all four natural deoxynucleoside triphosphates, 4 μCi [α-³²P]dCTP, 5 mM MgCl₂, and increasing concentrations of PSI-7409 (up to 1 mM), D-ddFCTP, or aphidicolin. DNA polymerase α, β, or γ is added to the reaction mixture to give final concentrations of 20, 18, and, 50 μg/mL, respectively. All reactions are run at 37°C and quenched at 30 min by mixing with 1 μL of 0.5 M EDTA. The radiolabeled products are quantified. A nonlinear fit is performed to determine the IC₅₀. The activity of RNA polymerase II is determined in a 25-μL in vitro transcription reaction mixture containing 100 ng of cytomegalovirus (CMV) immediate-early promoter DNA, 400 μM ATP, CTP, and UTP, 16 μM GTP, 10 μCi [α-³²P]GTP, 3 mM MgCl₂, and various concentrations of PSI-7409 (up to 1 mM), 3'-dCTP, or α-amanitin in transcription buffer (20 mM HEPES [pH 7.9], 100 mM KCl, 0.2 mM EDTA, 0.5 mM DTT, and 20% glycerol). All reactions are run at 30°C and quenched at 60 min by mixing with 125 μL of stop solution (0.3 M Tris-HCl [pH 7.4], 0.3 M sodium acetate, 0.5% SDS, 2 mM EDTA, and 3 μg/mL tRNA). The RNA product is purified. The resulting samples contains 12 μL and the same volume of gel loading dye (98% formamide, 10 mM EDTA, 0.1% xylene cyanol, and 0.1% bromophenol blue) is added. The samples are heated at 90°C for 5 min and loaded onto a 6% polyacrylamide sequencing gel. After running, the gel is exposed to a phosphorscreen, and the product is visualized and quantified by using a phosphorimager^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Lam AM, et al. PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96. [Content Brief]

[2]. Murakami E, et al. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. J Biol Chem. 2010 Nov 5;285(45):34337-47. [Content Brief]

PSI-7409 tetrasodium 相关分类

Infection

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.7004 mL	8.5021 mL	17.0042 mL	42.5105 mL
	5 mM	0.3401 mL	1.7004 mL	3.4008 mL	8.5021 mL
	10 mM	0.1700 mL	0.8502 mL	1.7004 mL	4.2510 mL
	15 mM	0.1134 mL	0.5668 mL	1.1336 mL	2.8340 mL
	20 mM	0.0850 mL	0.4251 mL	0.8502 mL	2.1255 mL
	25 mM	0.0680 mL	0.3401 mL	0.6802 mL	1.7004 mL
	30 mM	0.0567 mL	0.2834 mL	0.5668 mL	1.4170 mL
	40 mM	0.0425 mL	0.2126 mL	0.4251 mL	1.0628 mL
	50 mM	0.0340 mL	0.1700 mL	0.3401 mL	0.8502 mL
	60 mM	0.0283 mL	0.1417 mL	0.2834 mL	0.7085 mL
	80 mM	0.0213 mL	0.1063 mL	0.2126 mL	0.5314 mL
	100 mM	0.0170 mL	0.0850 mL	0.1700 mL	0.4251 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

PSI-7409 tetrasodium1621884-22-7HCVHepatitis C virusInhibitorinhibitorinhibit

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MedChemExpress (MCE) 只为有资质的科研机构、医药企业基于科学研究或药证申报的用途提供医药研发服务，不为任何个人或者非科研性质的、非用于药证申报使用等其他用途提供服务。

站点地图隐私声明

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上海工商

沪公网安备31011502019417

沪(浦)应急管危经许[2021]201709(QFYS)

营业执照（三证合一）

PSI-7409 tetrasodium

Customer Review

Other Forms of PSI-7409 tetrasodium:

MCE 顾客使用本产品发表的 6 篇科研文献