2. Academic Validation
  3. Light-controlled gene silencing in zebrafish embryos

Light-controlled gene silencing in zebrafish embryos

  • Nat Chem Biol. 2007 Oct;3(10):650-1. doi: 10.1038/nchembio.2007.30.
Ilya A Shestopalov 1,  Surajit Sinha,  James K Chen
Affiliations

Affiliation

  • 1 Department of Chemical and Systems Biology, Stanford University School of Medicine, 269 Campus Drive, CCSR 3155, Stanford, California 94305, USA.
PMID: 17717538 DOI: 10.1038/nchembio.2007.30
Abstract

Functional genomic studies in zebrafish frequently use synthetic Oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.

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